Whole-mount confocal microscopy for vascular branching morphogenesis.

Methods Mol Biol

Laboratory of Stem Cell and Neuro-Vascular Biology, Genetics and Developmental Biology Center, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.

Published: April 2012

We introduce a whole-mount immunohistochemistry method for analyzing intricate vascular network formation in mouse embryonic tissues. Laser scanning confocal microscopy with multiple labeling allows for robust imaging of blood and lymphatic vessel branching morphogenesis with excellent resolution.

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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199634PMC
http://dx.doi.org/10.1007/978-1-61779-523-7_7DOI Listing

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