We introduce a whole-mount immunohistochemistry method for analyzing intricate vascular network formation in mouse embryonic tissues. Laser scanning confocal microscopy with multiple labeling allows for robust imaging of blood and lymphatic vessel branching morphogenesis with excellent resolution.
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC4199634 | PMC |
http://dx.doi.org/10.1007/978-1-61779-523-7_7 | DOI Listing |
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