Today, matrix-based screens are used primarily for smaller and medium-size clone collections in combination with automation and cloning techniques that allow for reliable and fast interaction screening. Matrix-based yeast two-hybrid screens are an alternative to library-based screens. However, intermediary forms are possible too and we compare both strategies, including a detailed discussion of matrix-based screens. Recent improvement of matrix screens (also called array screens) uses various pooling strategies as well as novel vectors that increase their efficiency while decreasing false-negative rates and increasing reliability.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/978-1-61779-455-1_1 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Preparation of Hybrid Sol-Gel Materials Based on Living Cells of Microorganisms and Their Application in Nanotechnology.
Nanomaterials (Basel)
March 2022
N. D. Zelinsky Institute of Organic Chemistry, Leninsky pr. 47, 119991 Moscow, Russia.
Microorganism-cell-based biohybrid materials have attracted considerable attention over the last several decades. They are applied in a broad spectrum of areas, such as nanotechnologies, environmental biotechnology, biomedicine, synthetic chemistry, and bioelectronics. Sol-gel technology allows us to obtain a wide range of high-purity materials from nanopowders to thin-film coatings with high efficiency and low cost, which makes it one of the preferred techniques for creating organic-inorganic matrices for biocomponent immobilization.
View Article and Find Full Text PDFNovel xylose transporter Cs4130 expands the sugar uptake repertoire in recombinant strains at high xylose concentrations.
Biotechnol Biofuels
August 2020
Brazilian Biorenewable National Laboratory (LNBR), Brazilian Center for Research in Energy and Materials (CNPEM), Campinas, São Paulo 13083-100 Brazil.
Background: The need to restructure the world's energy matrix based on fossil fuels and mitigate greenhouse gas emissions stimulated the development of new biobased technologies for renewable energy. One promising and cleaner alternative is the use of second-generation (2G) fuels, produced from lignocellulosic biomass sugars. A major challenge on 2G technologies establishment is the inefficient assimilation of the five-carbon sugar xylose by engineered strains, increasing fermentation time.
View Article and Find Full Text PDFPredicting Protein-Protein Interactions from Matrix-Based Protein Sequence Using Convolution Neural Network and Feature-Selective Rotation Forest.
Sci Rep
July 2019
School of information engineering, JiangXi University of Science and Technology, Ganzhou, Jiangxi, 341000, P.R. China.
Protein is an essential component of the living organism. The prediction of protein-protein interactions (PPIs) has important implications for understanding the behavioral processes of life, preventing diseases, and developing new drugs. Although the development of high-throughput technology makes it possible to identify PPIs in large-scale biological experiments, it restricts the extensive use of experimental methods due to the constraints of time, cost, false positive rate and other conditions.
View Article and Find Full Text PDFUricase grafted nanoconducting matrix based electrochemical biosensor for ultrafast uric acid detection in human serum samples.
Int J Biol Macromol
June 2019
Academy of Scientific and Innovative Research (AcSIR), CSIR - National Physical Laboratory (Campus), New Delhi 110012, India; CSIR - National Physical Laboratory, New Delhi 110012, India. Electronic address:
Gold nanoparticles decorated graphene oxide (Au-rGO) nanocomposite thin films with enhanced electro-active characteristics were prepared and covalently immobilized with uricase (UOx) enzyme for sensitive and selective detection of uric acid (UA). Differential pulse voltammetry (DPV) studies revealed rapid response of fabricated electrode towards UA at low potential (0.228 V) in a wide concentration range of 50-800 μM with a sensitivity of 86.
View Article and Find Full Text PDFAntimicrobial effect of novel hydrogel matrix based on natural polysaccharide Sterculia urens.
Epidemiol Mikrobiol Imunol
April 2019
Introduction: Materials for modern wound-management are a very broad and heterogeneous group. One of the most important representatives is natural materials, or more precisely polysaccharides isolated from various plants and animals. With the increasing resistance of pathogens to established antimicrobial agents, there is also an attempt to discover new mechanisms of the effects of these materials.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!