Protein adsorption onto capillary wall often hampers CE separations, particularly in the CZE mode. Electrostatic interactions are not the only factors affecting adsorption, as hydrophobic interactions and/or protein conformational changes are also involved in the adsorption phenomenon. Numerous methods can be used to reduce or avoid adsorption, such as (i) addition of low molecular weight molecules in the BGE, (ii) use of surfactants, or (iii) capillary coatings. However, most of these methods are not MS-compatible. In this study, we evaluated the addition of organic solvent as an alternative MS-compatible method to decrease protein adsorption. The effect of the solvent addition was emphasized using classical methods for estimating reversible and irreversible adsorption. In many cases, organic solvents were effective at decreasing adsorption. However, the influence of the organic solvent on protein adsorption should be evaluated case-by-case in CE method development.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1002/elps.201000245 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Oyster Shell Powder/PCL Composite Scaffolds Loaded with Psoralen Nanospheres Promote Large Bone Defect Repair.
ACS Omega
January 2025
Shaanxi University of Chinese Medicine, Xianyang 712046, China.
Research on bone substitutes for repairing bone defects has drawn increasing attention, and the efficacy of three-dimensional (3D) printed bioactive porous scaffolds for bone defect repair has been well documented. Our previous studies have shown that psoralen can promote osteogenesis by activating the Wnt/β-catenin and BMP/Smad signaling pathways and their crosstalk effects, and psoralen nanospheres have a good osteogenesis-promoting effect with low cytotoxicity. The Chinese medicine oyster shell powder, characterized by its porous structure, strong adsorption, and unique bioactivity, has potential in fracture-promoting repair materials.
View Article and Find Full Text PDFOptimized Synthesis and Stabilization of Superparamagnetic Iron Oxide Nanoparticles for Enhanced Biomolecule Adsorption.
ACS Omega
January 2025
Federal University of Espírito Santo, Av Marechal Campos 1468, Vitória, ES 29.040 090, Brazil.
Monodisperse and colloidally stable magnetic iron oxide nanoparticles have been developed for diverse biotechnology applications. Although promising for the adsorption of organic molecules, the low density of adsorption sites in these nanoparticles has been a significant challenge. In this study, an optimized factorial design with response surface methodology (RSM) was employed to produce small Superparamagnetic Iron Oxide Nanoparticles (SPIONs) stabilized with tetraethoxysilane (TEOS).
View Article and Find Full Text PDFOne-Step Synthesis of Carboxylated Polyethylene Glycol-Modified Polystyrene Microspheres and Their Application in the Luminescent Oxygen Channel Immunoassay.
Langmuir
January 2025
School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China.
As one of the key diagnostic methods for detecting biomarkers and antigen-antibody interactions, the luminescent oxygen channel immunoassay (LOCI) has been widely applied in bioanalysis and other fields. In the context of LOCI, the performance of the prepared donor polystyrene (PS) microspheres significantly impacts the detection signal values. In this study, an attempt was made to synthesize PS microspheres via one-step polymerization of styrene with an amphiphilic monomer (PEOOH), followed by swelling the silicon phthalocyanine photosensitizer into the PS microspheres, resulting in the functionalization of the PS microspheres with polyethylene glycol segments.
View Article and Find Full Text PDFUtilizing 4-Sulfonylcalix[4]arene as a Selective Mobile Phase Additive for the Capture of Methylated Peptides.
Anal Chem
January 2025
Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Meilong Road, Shanghai 200237, P. R. China.
Protein methylation has attracted increasing attention due to its significant regulatory roles in various biological processes. However, the diversity of methylation forms, subtle differences between methylated and nonmodified sites, and their ultralow abundances pose substantial challenges for capturing and isolating methylated peptides from biological samples. Herein, we develop a chromatographic method that utilizes 4-sulfonylcalix[4]arene (SC4A) as a mobile phase additive and Click-Maltose as the stationary phase to separate methylated/nonmethylated peptides through the adsorption of the SC4A-(Me3) complex.
View Article and Find Full Text PDFGold nanorod in silver tetrahedron: Cysteamine mediated synthesis of SERS probes with embedded internal markers for AFP detection.
Anal Chim Acta
February 2025
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, School of Life Science and Technology, Xi'an Jiaotong University, No. 28 Xianning West Road, Xi'an, 710049, China. Electronic address:
Background: Plasmonic core-shell nanostructures with embedded internal markers used as Raman probes have attracted great attention in surface-enhanced Raman scattering (SERS) immunoassay for cancer biomarkers due to their excellent uniform enhancement. However, current core-shell nanostructures typically exhibit a spherical shape and are coated with a gold shell, resulting in constrained local field enhancement.
Results: In this work, we prepared a core-shell AuNR@BDT@Ag structure by depositing silver on the surface of Raman reporter-modified gold nanorods (AuNR).
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!