AI Article Synopsis
- This study focuses on improving glucosamine (GlcN) production through microbial cells, specifically using Citrobacter sp. BL-4, which has been shown to produce polyglucosamine.
- Researchers conducted LC-MS analysis after protein separation to identify proteins involved in acetate metabolism, discovering 280 proteins, of which 188 were related to acetate.
- Key findings include the up-regulation of acetyl-CoA synthetase and isocitrate lyases in acetate medium, leading to significant changes in metabolic pathways that could enhance GlcN production through targeted metabolic engineering.
Article Abstract
Mass production of glucosamine (GlcN) using microbial cells is a worthy approach to increase added values and keep safety problems in GlcN production process. Prior to set up a microbial cellular platform, this study was to assess acetate metabolism in Citrobacter sp. BL-4 (BL-4) which has produced a polyglucosamine PGB-2. The LC-MS analysis was conducted after protein separation on the 1D-PAGE to accomplish the purpose of this study. 280 proteins were totally identified and 188 proteins were separated as acetate-related proteins in BL-4. Acetate was converted to acetyl-CoA by acetyl-CoA synthetase up-regulated in the acetate medium. The glyoxylate bypass in the acetate medium was up-regulated with over-expression of isocitrate lyases and 2D-PAGE confirmed this differential expression. Using (1)H-NMR analysis, the product of isocitrate lyases, succinate, increased about 15 times in the acetate medium. During acetate metabolism proteins involved in the lipid metabolism and hexosamine biosynthesis were over-expressed in the acetate medium, while proteins involved in TCA cycle, pentose phosphate cycle and purine metabolism were down-regulated. Taken together, the results from the proteomic analysis can be applied to improve GlcN production and to develop metabolic engineering in BL-4.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3248649 | PMC |
| http://dx.doi.org/10.7150/ijbs.8.66 | DOI Listing |
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