Modified charcoal-cefoperazone-deoxycholate agar (mCCDA) was improved by supplementation with a high concentration of polymyxin B. The ability of the supplemented medium to isolate Campylobacter jejuni and C. coli from chicken carcass rinses was compared to that of Campy-Cefex agar and mCCDA. Modification of mCCDA with increased polymyxin B yielded a significantly (P < 0.05) higher isolation rate and greater selectivity than those achieved using Campy-Cefex agar and mCCDA.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3294483 | PMC |
| http://dx.doi.org/10.1128/AEM.07180-11 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
sp. nov., isolated from caecal contents of domestic pigs ().
Int J Syst Evol Microbiol
October 2023
University of Ljubljana, Veterinary Faculty, Institute of Microbiology and Parasitology, Ljubljana, Slovenia.
During the 2021 European Food Safety Authority coordinated harmonized monitoring of antimicrobial resistance in species in Slovenia, five -like strains were cultured from caeca of a total of 104 domestic pigs that could not be identified using the standard-prescribed biochemical tests or MALDI-TOF MS. The isolates were obtained using the standard ISO 10272 procedure for the isolation of thermotolerant with prolonged cultivation time. Small -like colonies were observed on mCCDA and CASA agar plates after 2-4 days of incubation; dark-field microscopy revealed relatively big spirilli-shaped bacteria exhibiting characteristic -like motility.
View Article and Find Full Text PDFInvestigating the Significance of Non-/ Strains in Patients with Diarrhea.
Healthcare (Basel)
September 2023
Medical Microbiology Department, Istanbul Faculty of Medicine, Istanbul University, 34093 Istanbul, Turkey.
is one of the most commonly reported foodborne bacteria worldwide. Although and have been reported to be responsible for the great majority of campylobacteriosis, the burden of infections by species other than and have been increasing as a result of a transition to diagnostic test methods that enable the isolation of emerging species. The aim of the present study was to recover , , and emerging species from the stool samples of 500 patients with gastroenteritis and 100 healthy subjects via the use of a filtration method and culture techniques using Butzler agar and mCCDA under a microaerobic or hydrogen-enriched atmosphere, identify the species by multiplex PCR methods and assess the significance of emerging species in enteric diseases.
View Article and Find Full Text PDFComparison of Two Culture-Based Detection Systems for the Isolation of Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii in Raw Ground Poultry.
J Food Prot
March 2023
R & F Products, Inc., 2725 Curtiss Street, Downers Grove, IL 60188, USA.
Arcobacters are emerging pathogens that have been underestimated due to a lack of a standardized isolation method. The aim of this research was to evaluate the ability to isolate Arcobacter butzleri, Arcobacter cryaerophilus, and Arcobacter skirrowii using two Arcobacter-specific culture detection systems: (i) the Houf broth and modified charcoal cefoperazone deoxycholate agar supplemented with cefoperazone, amphotericin B, and teicoplanin (HB/mCCDA+CAT), and (ii) the Nguyen-Restaino-Juárez Arcobacter enrichment broth and chromogenic agar (NRJ-B/M). Both detection systems were evaluated for productivity ratio, sensitivity, and specificity.
View Article and Find Full Text PDFsp. nov., isolated from caecal contents of domestic pigs ().
Int J Syst Evol Microbiol
November 2022
University of Ljubljana, Veterinary Faculty, Institute of Microbiology and Parasitology, Ljubljana, Slovenia.
Seven -like isolates were cultured from caecal contents of 100 domestic pigs () sampled as part of the EFSA-coordinated harmonized monitoring of antimicrobial resistance in sp. in 2015. The bacteria were isolated using the standard ISO 10272 procedure for the isolation of thermotolerant with extended incubation time and formed small, grey, moist and flat colonies with a metallic sheen (small -like colonies) on modified Charcoal-Cefoperazone-Deoxycholate Agar (mCCDA) and Skirow agar plates.
View Article and Find Full Text PDFWild boars as reservoir for Campylobacter and Arcobacter.
Vet Microbiol
July 2022
Department of Veterinary and Biosciences, Faculty of Veterinary Medicine, Ghent University, Heidestraat 19, Merelbeke, Belgium; Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, Karel Lodewijk Ledeganckstraat 35, 9000 Ghent, Belgium. Electronic address:
Article Synopsis
- Campylobacteriosis is a public health issue mainly caused by Campylobacter jejuni and Campylobacter coli, but there is growing awareness of other species like Arcobacter; however, little is known about Arcobacter in wild boars.
- A study examined fecal samples from 76 wild boars in Italy using various isolation techniques, finding that over half of the animals carried Campylobacter or Arcobacter, with Campylobacter lanienae being the most common.
- Almost 40% of the boars had high levels of Campylobacter in their feces, raising concerns about potential meat contamination, especially since these animals were hunted for consumption.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!