Production of oils from acetic acid by the oleaginous yeast Cryptococcus curvatus.

Appl Biochem Biotechnol

Laboratoire de Génie Chimique et Biochimique, Polytech Clermont-Ferrand, Clermont Université, Université Blaise Pascal, 24 Av. des Landais, BP 20206, 63174 Aubière, France.

Published: July 2012

AI Article Synopsis

  • A study found that the yeast Cryptococcus curvatus can convert acetic acid into oils, but it grows slowly on acetate alone, leading to a two-step process for improved efficiency.
  • The first step involves growing the yeast on glucose until it's used up, and the second step uses acetate with limited nitrogen to boost lipid production.
  • Experiments showed that this method can lead to a lipid accumulation of up to 50%, indicating it is a viable technique for oil production.

Article Abstract

The feasibility of the conversion of acetic acid, a metabolite commonly obtained during anaerobic fermentation processes, into oils using the yeast Cryptococcus curvatus was reported. This microorganism exhibited very slow growth rates on acetate as carbon source, which led to design a two-stage cultivation process. The first consisted of cell growth on glucose as carbon source until its complete exhaustion. The second step involved the use of acetate as carbon source under nitrogen limitation in order to induce lipid accumulation. A typical experiment performed in a bioreactor involved a preliminary yeast growth with a glucose initial concentration of 15 g/L glucose. Further additions of acetate and nitrogen source allowed a final lipid accumulation up to 50% (w/w). These promising results demonstrated the suitability of the technique proposed.

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Source
http://dx.doi.org/10.1007/s12010-011-9507-5DOI Listing

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