During left-right (L-R) axis formation, Nodal is expressed in the node and has a central role in the transfer of L-R information in the vertebrate embryo. Bone morphogenetic protein (BMP) signaling also has an important role for maintenance of gene expression around the node. Several members of the Cerberus/Dan family act on L-R patterning by regulating activity of the transforming growth factor-β (TGF-β) family. We demonstrate here that chicken Dan plays a critical role in L-R axis formation. Chicken Dan is expressed in the left side of the node shortly after left-handed Shh expression and before the appearance of asymmetrically expressed genes in the lateral plate mesoderm (LPM). In vitro experiments revealed that DAN inhibited BMP signaling but not NODAL signaling. SHH had a positive regulatory effect on Dan expression while BMP4 had a negative effect. Using overexpression and RNA interference-mediated knockdown strategies, we demonstrate that Dan is indispensable for Nodal expression in the LPM and for Lefty-1 expression in the notochord. In the perinodal region, expression of Dan and Nodal was independent of each other. Nodal up-regulation by DAN required NODAL signaling, suggesting that DAN might act synergistically with NODAL. Our data indicate that Dan plays an essential role in the establishment of the L-R axis by inhibiting BMP signaling around the node.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ydbio.2011.12.015 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
ET-3/ETBR Mediates Na-Activated Immune Signaling and Kidney Lymphatic Dynamics.
Circ Res
December 2024
Department of Pediatrics, Vanderbilt University Medical Center, Nashville, TN. (J.Z., H.-C.Y., R.L.R., E.L.S., V.K.).
Background: Lymphatic collecting vessels in the kidney are critical in clearing interstitial fluid, macromolecules, and infiltrating immune cells. Dysfunction of the lymphatic vessels can disrupt this process and exacerbate injury-associated inflammation in many disease conditions. We previously found that sodium accumulates within the kidney interstitium during proteinuric kidney injury and elevated sodium environments stimulate isolevuglandin production in antigen-presenting cells, stimulating T cells, and modulating inflammatory responses.
View Article and Find Full Text PDFTeneurin C-Terminal Associated Peptide (TCAP)-1 Attenuates Restraint Stress-Induced Corticosterone Increases in Male Mice and Rats.
Pharmacol Res Perspect
December 2024
Protagenic Therapeutics Inc., New York, New York, USA.
Hyperactivation of the hypothalamic-pituitary-adrenal (HPA) axis response can result in anxiety and other neuropsychiatric disorders and effective therapeutics are needed to mitigate this maladaptive response. Here we examined the effects of Teneurin C-terminal Associated Peptide (TCAP)-1, a peptide known to inhibit corticotropin releasing factor (CRF)-mediated stress, on the physiological expression of stress, and whether the effects of TCAP-1 were dependent on the route of administration. We first examined whether subcutaneous administration of TCAP-1 influenced tube restraint stress-induced corticosterone (CORT) increases in both male mice and rats.
View Article and Find Full Text PDFAccelerated Cardiac MRI with Deep Learning-based Image Reconstruction for Cine Imaging.
Radiol Cardiothorac Imaging
December 2024
From the Institute of Diagnostic and Interventional Radiology, Pediatric Radiology and Neuroradiology (A.C.K., L.R., M.M., A.D., M.A.W., F.G.M.), and Department of Cardiology (C.I.L.), Rostock University Medical Center, Schillingallee 36, 18057 Rostock, Germany; GE HealthCare, Munich, Germany (M.G.); GE HealthCare, Menlo Park, Calif (X.Z.); and Department of Radiology, Ludwig-Maximilian University, Munich, Germany (R.L.).
Purpose To assess the influence of deep learning (DL)-based image reconstruction on acquisition time, volumetric results, and image quality of cine sequences in cardiac MRI. Materials and Methods This prospective study (performed from January 2023 to March 2023) included 55 healthy volunteers who underwent a noncontrast cardiac MRI examination at 1.5 T.
View Article and Find Full Text PDFHyperglycaemia induces diet-dependent defects of the left-right axis by lowering intracellular pH.
Biochim Biophys Acta Mol Basis Dis
January 2025
Department of Developmental Biology, Graduate School of Medical Sciences, Kyushu University, Fukuoka 812-8582, Japan. Electronic address:
Article Synopsis
- * Analysis of mouse embryos from diabetic mothers showed disruptions in the expression of genes related to left-right (L-R) axis formation, particularly Pitx2 and Nodal.
- * High glucose levels affected metabolic processes and signaling pathways in embryos, and maternal vitamin A intake was found to worsen L-R axis defects, highlighting the role of diet in preventing such anomalies in diabetic pregnancies.
Patient-Specific Rods in Adolescent and Adult Spinal Deformity Surgery: A Narrative Review.
Int J Spine Surg
August 2024
Department of Orthopedic Surgery, Columbia University Medical Center, NewYork-Presbyterian Och Spine Hospital, New York, NY, USA
Spinal deformity surgery often requires complex surgical interventions that can have a drastic effect on both patient quality of life and functional capacity. Modern-day corrective solutions for these deformities include spinal osteotomies, pedicle screw instrumentation, and dual/multirod constructs. These solutions are efficacious and are currently considered standard practice for spinal surgeons, but they lack individualization.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!