AI Article Synopsis
- The intestinal epithelium relies on stem cells at the base of crypts to maintain cell turnover, with the study focusing on their roles in cell proliferation and apoptosis in the terminal ileum and various colon regions.
- Under normal conditions, the ascending colon exhibited the smallest crypt size and had a similar pattern of proliferating cells to that of the terminal ileum, while post-radiation, apoptotic cells were primarily found at the crypt's base in the mid and descending colon but spread more evenly in the ascending colon.
- The findings indicate that stem cell locations in both the ascending colon and terminal ileum share similarities in their profiles of cell proliferation and apoptosis, particularly after radiation exposure.
Article Abstract
Purpose: The intestinal epithelium has a high rate of cell turnover, which is regulated by stem cells located near the base of crypts. We aimed to investigate stem cell-dependent characteristics of cell proliferation, apoptosis, and crypt size in terminal ileum and different regions of the colon.
Methods And Materials: Mice were studied under steady-state conditions and after radiation-induced stem cell apoptosis. Percentage of proliferating or apoptotic cells at a particular cell position (cp) along the crypt axis was expressed as labeling or apoptotic index.
Results: Under steady-state conditions: crypt size was smallest in the ascending colon. In contrast to other regions of the colon, the distribution profile of proliferating cells in ascending colon showed some similarity to that in the terminal ileum. Postirradiation: apoptotic cells were prominent at the bottom of the crypt of mid- and descending colon but in the ascending colon, they were seen with similar frequency from cp 1 to 4. During regeneration, a constant proliferative capacity was seen above Paneth cells in the terminal ileum. In the ascending (but not mid- or descending) colon, the profile of proliferating cells over the first 4 days after irradiation showed a similarity to that in the terminal ileum.
Conclusions: Profiles of proliferating epithelial cells (under steady-state conditions and postirradiation) and apoptotic cells (postirradiation) suggest similarities in the location of stem cells in the ascending colon and terminal ileum.
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| http://dx.doi.org/10.1016/j.ijrobp.2011.07.015 | DOI Listing |
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