AI Article Synopsis
- The application of a voltage ramp causes denaturation of double-stranded DNA (dsDNA), leading to strand separation.
- The melting potential (E(m)), at which half of the dsDNA duplexes denature, is shown to correlate with the calculated nearest neighbor and experimental melting temperatures (T(m)).
- This correlation suggests that electrochemical melting potential can be used to assess dsDNA stability, allowing for the design of DNA probes with predictable melting potentials for future experiments.
Article Abstract
Application of a voltage ramp can result in denaturation of dsDNA and strand separation. We show that the potential at which half of the surface immobilised duplexes denature (the melting potential, E(m)) directly correlates with the calculated nearest neighbour and experimental melting temperatures; T(m), for a duplex is solution. The results demonstrate that the electrochemical melting potential measures the stability of the dsDNA, and therefore existing nearest neighbour melting models can be utilized to design DNA probes with predictable electrochemical melting potentials for future assay applications.
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| http://dx.doi.org/10.1016/j.bioelechem.2011.11.003 | DOI Listing |
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