Aim: Oocyte cryopreservation remains largely experimental, with live birth rates of only 2-4% per thawed oocyte. In this study, we present a nanoliter droplet technology for oocyte vitrification.
Materials & Methods: An ejector-based droplet vitrification system was designed to continuously cryopreserve oocytes in nanoliter droplets. Oocyte survival rates, morphologies and parthenogenetic development after each vitrification step were assessed in comparison with fresh oocytes.
Results: Oocytes were retrieved after cryoprotectant agent loading/unloading, and nanoliter droplet encapsulation showed comparable survival rates to fresh oocytes after 24 h in culture. Also, oocytes recovered after vitrification/thawing showed similar morphologies to those of fresh oocytes. Additionally, the rate of oocyte parthenogenetic activation after nanoliter droplet encapsulation was comparable with that observed for fresh oocytes. This nanoliter droplet technology enables the vitrification of oocytes at higher cooling and warming rates using lower cryoprotectant agent levels (i.e., 1.4 M ethylene glycol, 1.1 M dimethyl sulfoxide and 1 M sucrose), thus making it a potential technology to improve oocyte cryopreservation outcomes.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3319864 | PMC |
| http://dx.doi.org/10.2217/nnm.11.145 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Droplet-Based EPR Spectroscopy for Real-Time Monitoring of Liquid-Phase Catalytic Reactions.
Small Methods
January 2025
Institute of Chemical and Bioengineering, Department of Chemistry and Applied Biosciences, ETH Zürich, Vladimir-Prelog-Weg 1, Zürich, 8093, Switzerland.
In situ monitoring is essential for catalytic process design, offering real-time insights into active structures and reactive intermediates. Electron paramagnetic resonance (EPR) spectroscopy excels at probing geometric and electronic properties of paramagnetic species during reactions. Yet, state-of-the-art liquid-phase EPR methods, like flat cells, require custom resonators, consume large amounts of reagents, and are unsuited for tracking initial kinetics or use with solid catalysts.
View Article and Find Full Text PDFRapid High-Throughput Discovery of Molecules With Antimicrobial Activity From Natural Products Enabled by a Nanoliter Matrix SlipChip.
Small Methods
January 2025
School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, 200030, China.
Article Synopsis
- Improper use of antibiotics is creating "superbugs" that resist treatment, outpacing new antibiotic discovery.
- A new screening method using a nanoliter matrix SlipChip (nm-SlipChip) allows for rapid testing of potential antibiotics, reducing sample consumption significantly.
- This method successfully identified two novel compounds from the plant Callicarpa integerrima that can combat MRSA, highlighting its potential in tackling antibiotic resistance.
Automated Nanoliter Volume Assay Optimization on a Cost-Effective Microfluidic Disc.
Anal Chem
January 2025
Department of Chemistry, University of Virginia, Charlottesville, Virginia 22904, United States.
Optimizing multireagent assays often requires successive titration of individual components until the optimal combination of conditions is achieved. This process is time-consuming, laborious, and often expensive since parallelized experimentation requires bulk consumption of reagents. Microfluidics presents a solution through miniaturization of standard processes by reducing reaction volume, executing multiple parallel workflows, and enabling automation.
View Article and Find Full Text PDFPermeability-Engineered Compartmentalization System Promises Next-Generation Single-Cell Analysis.
Anal Chem
December 2024
HKUST Fok Ying Tung Research Institute, Guangzhou 511458, China.
Single-cell analysis, including sequencing, imaging, and biochemical assays, has become a fundamental strategy in biomedical research. Microplates, with their open system design, facilitate multistep reagent addition, subtraction, and buffer exchange, while their physically isolated wells prevent cross-contamination between biomolecules, establishing them as foundational compartmentalized platform for single-cell analysis. In contrast, water-in-oil droplets, produced by microfluidic systems, create nanoliter/picoliter-sized droplets that act as advanced compartmentalized platform.
View Article and Find Full Text PDFDroplet-based microprocessors.
Science
November 2024
Centre de Recherche Paul Pascal, University of Bordeaux, CNRS, CRPP-UMR5031, Pessac, France.
Soft devices made of nanoliter hydrogel beads use ions for computation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!