The expression of two cardiac myosin heavy chain (MyHC) isoforms in response to the thyroid status was studied in left ventricles (LVs) of Lewis rats. Major MyHC isoform in euthyroid and hyperthyroid LVs had a higher mobility on SDS-PAGE, whereas hypothyroid LVs predominantly contained a MyHC isoform with a lower mobility corresponding to that of the control soleus muscle. By comparing the MyHC profiles obtained under altered thyroid states together with the control soleus, we concluded that MyHCα was represented by the lower band with higher mobility and MyHCβ by the upper band. The identity of these two bands in SDS-PAGE gels was confirmed by western blot and mass spectrometry. Thus, in contrast to the literature data, we found that the MyHCα possessed a higher mobility rate than the MyHCβ isoform. Our data highlighted the importance of the careful identification of the MyHCα and MyHCβ isoforms analyzed by the SDS-PAGE.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3237020 | PMC |
| http://dx.doi.org/10.1155/2011/634253 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Relationship between multiple morbidities and performance on the Timed Up and Go test in elderly patients: a cross-sectional study.
BMJ Open
January 2025
University of Social Welfare and Rehabilitation Science, Tehran, Iran (the Islamic Republic of).
Objective: To investigate how various morbidities affect older patients' performance on the Timed Up and Go (TUG) test.
Design: Cross-sectional study.
Setting: The seven government hospitals of Lahore, Pakistan, included are major tertiary care centres, representing an older patient population of Punjab, Pakistan.
Risk quantification based Adaptive Cruise control and its application in approaching behavior at signalized intersections.
Accid Anal Prev
January 2025
School of Transportation, Southeast University, Nanjing, Jiangsu Province 211189, PR China; Institute on Internet of Mobility, Southeast University and University of Wisconsin-Madison, Southeast University, Nanjing, Jiangsu Province 211189, PR China.
Traffic signals, while reducing conflicts within intersections, often lead to stop-and-go behaviors in approaching vehicles, negatively impacting traffic flow in terms of safety, efficiency, and fuel consumption. Aimed at minimizing the traffic oscillations caused by traffic signals through Connected and Autonomous Vehicles (CAVs) and meeting real-time operational needs, this paper proposes a Risk-Based Adaptive Cruise Control (RACC). RACC designs the constraints of approaching a signalized intersection as expected risks, enabling compliance with all constraints while being adaptable to basic road scenarios.
View Article and Find Full Text PDFStudy on the impact of Kinesiophobia after Total Knee Arthroplasty on the rehabilitation of patients during hospitalization: A pilot study.
PLoS One
January 2025
Department of Operating Room, Baoding First Central Hospital, Baoding, China.
The purpose of this study is to investigate the influence of kinesiophobia following Total Knee Arthroplasty (TKA) on the rehabilitation outcomes of patients during hospitalization, includes examining the trends in resting pain levels at various time points post-surgery, the trends in active flexion of the knee at various time points post-surgery, and the effects of kinesiophobia on the timing of first postoperative ambulation, the duration of postoperative hospital stay, and the results of the two-minute walk test on the day of discharge. Postoperative kinesiophobia in patients was identified using the Tampa Scale for Kinesiophobia (TSK), with 33 patients scoring >37 points and 35 patients scoring ≤37 points. Resting Pain levels were assessed using the Numerical Rating Scale (NRS) at various time points, including upon return to the ward (T1), the first (T2), second (T3), third (T4), fifth(T5) postoperative days, and the day of discharge (T6).
View Article and Find Full Text PDFSize-Dependent Reduction Kinetics of Iron Oxides in Single and Mixed Mineral Systems.
Environ Sci Technol
January 2025
Geomicrobiology, Department of Geosciences, University of Tübingen, 72076 Tübingen, Germany.
Iron(III) (oxyhydr)oxide minerals with varying particle sizes commonly coexist in natural environments and are susceptible to both chemical and microbial reduction, affecting the fate and mobility of trace elements, nutrients, and pollutants. The size-dependent reduction behavior of iron (oxyhydr)oxides in single and mixed mineral systems remains poorly understood. In this study, we used microbial and mediated electrochemical reduction approaches to investigate the reduction kinetics and extents of goethite and hematite.
View Article and Find Full Text PDFImine-Based Polymeric Mixed Ionic-Electronic Conductors Featuring Degradability and Biocompatibility for Transient Bioinspired Electronics.
Angew Chem Int Ed Engl
January 2025
Sun Yat-Sen University, School of Material Science and Engineering, Nr.135 Xingang Xi Road, 510275, Guangzhou, CHINA.
Degradable features are highly desirable to advance next-generation organic mixed ionic-electronic conductors (OMIECs) for transient bioinspired artificial intelligence devices.It is highly challenging that OMIECs exhibit excellent mixed ionic-electronic behavior and show degradability simultaneously.Specially,in OMIECs,doping is often a tradeoff between structural disorder and charge carrier mobilities.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!