In 2003-2010 5483 persons suspected of echinococcosis were examined for Echinococcus granulosus and Echinococcus multilocularis infections using serological tests in the Department of Medical Parasitology National Institute of Public Health - National Institute of Hygiene. The screening test ELISA (Bordier Affinity Products S. A.), confirmation tests Western-blot (LDBIO Diagnostics, Lyon-France) and ELISA Em2 plus (Bordier Affinity Products S.A) were applied. Within 235 persons posistive for in screening test, 162 were confirmed with Echinococcus granulosus and 33 - E. multilocularis. In 40 cases the tests failed to determine the Echinococcus species. In the period of 2006-2010 the linear decrease of percentage of positive results was observed. However, the information about the continuously growing population of red fox in Poland, together with the growing percentage of the animals infected with E. multilocularis, suggests the need for continuous monitoring of human alveococcosis.
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Pol J Vet Sci
December 2024
Pasteur Institute of Novi Sad, Hajduk Veljkova 1, 21101 Novi Sad, Serbia.
Constant antigenic changes, new variants and easy transmission of SARS-CoV-2 virus should acquire greater zoonotic attention and need to remain alert. In this retrospective study the aim was to analyze seropositivity to SARS-CoV-2 in dogs by commercial ELISA. The Virus neutralization test (VNT) was modified for the purpose of confirmation of SARS-CoV-2 antibodies in ELISA-positive dog sera.
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January 2024
Center for Research of Endemic Parasites of Iran, Tehran University of Medical Sciences, Tehran, Iran.
is an intracellular parasite capable of crossing the placenta in pregnancy and infecting the developing fetus, leading to various congenital anomalies and even abortion. Acute infection is responsible for almost all cases of congenital toxoplasmosis in immunocompetent pregnant women. Prenatal screening for acute toxoplasmosis primarily involves maternal serology and fetal ultrasound imaging.
View Article and Find Full Text PDFParasit Vectors
December 2024
College of Public Health, University of Iowa, Iowa City, IA, USA.
Background: Visceral leishmaniosis (VL) is the most severe form of human leishmaniosis, with an estimated 95% case fatality if left untreated. Dogs act as peridomestic reservoir hosts for the protozoan parasite Leishmania infantum, a causative agent for human leishmaniosis, endemic throughout the Mediterranean basin. To assure consistent and accurate surveillance of canine infection and prevent transmission to people, consistent diagnosis of canine L.
View Article and Find Full Text PDFBMC Vet Res
December 2024
Department of Parasitology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt.
Strongylus vulgaris, a devastating parasitic nematode in equids, causes life-threatening verminous aneurysms that are challenging to diagnose early. This study pioneered integrating nanotechnology into an indirect enzyme-linked immunosorbent assay (i-ELISA) system to enhance the sensitivity and specificity for detecting S. vulgaris larval antigens in equine serum samples, with PCR confirmation of the species.
View Article and Find Full Text PDFJ Microbiol Immunol Infect
December 2024
Center of Infectious Disease and Signaling Research, National Cheng Kung University, Tainan, Taiwan; Department of Pediatrics, National Cheng Kung University Hospital, College of Medicine, National Cheng Kung University, Tainan, 704, Taiwan; Department of Pediatrics, National Cheng Kung University Hospital Dou-Liou Branch, College of Medicine, National Cheng Kung University, Yunlin 640, Taiwan. Electronic address:
Background: Previously we identified a complex of non-structural protein (NS) 1 - Thrombin (NST) in dengue infected patients. Here, we investigated how the concentration of NS1 and NST differ in various dengue severity levels as well as their demographic and clinical features. Several comorbid (hypertension, diabetes, and chronic renal failure) often found in dengue patients were also measured and analyzed.
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