AI Article Synopsis
- Photoactivation localization microscopy (PALM) is used to investigate surface-enhanced fluorescence (SEF) on metal nanostructures, allowing for high-resolution imaging of individual molecules.
- Strong light scattering by metals changes the direction of emitted fluorescence, requiring adjustments in data analysis to account for spatial shifts relative to the nanostructures.
- SEF-PALM distinguishes effects of labeling density and can resolve nanostructures smaller than the diffraction limit, making it a valuable method for exploring plasmon-mediated effects on metal surfaces.
Article Abstract
Photoactivation localization microscopy (PALM) was applied to study surface-enhanced fluorescence (SEF) on metal nanostructures (SEF-PALM). The detection of fluorescence from individual single molecules can be used to image the point-spread-function and spatial distribution of the fluorescence emitted in the vicinity of a metal surface. Due to the strong scattering effect, the angular distribution of the fluorescence is altered by metals, resulting in a spatial shift of fluorescence spots with respect to the metal nanostructures, and has to be taken into account in the analysis. SEF-PALM can be used to discriminate effects of labelling density when estimating the enhancement factor in SEF. Furthermore, nanostructures with sizes below the diffraction limit can be resolved using this technique. SEF-PALM is established as a powerful tool to study plasmon-mediated phenomena on metal nanostructures.
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| http://dx.doi.org/10.1002/cphc.201100743 | DOI Listing |
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