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Activator-dependent acetylation of chromatin model systems. | LitMetric

Activator-dependent acetylation of chromatin model systems.

Methods Mol Biol

Department of Biochemistry and Molecular Biology, Colorado State University, Fort Collins, CO, USA.

Published: April 2012

AI Article Synopsis

  • Eukaryotic gene expression is closely linked to changes in chromatin structure in the nucleus, which are regulated by specific mechanisms.
  • To activate gene expression, transcriptional activators recruit histone acetyltransferases (HATs) to gene promoters, affecting histone tail modifications that can change chromatin architecture.
  • This study utilizes a model based on the human T-cell lymphoma virus type 1 promoter to explore how activator-dependent acetylation influences chromatin structure and RNA polymerase II transcription using various biochemical and biophysical techniques.

Article Abstract

Regulatory mechanisms underlying eukaryotic gene expression, and many other DNA metabolic pathways, are tightly coupled to dynamic changes in chromatin architecture in the nucleus. Activation of gene expression generally requires the recruitment of histone acetyltransferases (HATs) to gene promoters by sequence-specific DNA-binding transcriptional activators. HATs often target specific lysines in the core histone amino-terminal "tail" domains (NTDs), which have the potential ability to alter higher order chromatin structure. In order to better characterize the impact targeted histone acetylation has on chromatin structure and function, we have characterized a novel model system derived from the human T-cell lymphoma virus type 1 promoter. Using this system as an example, here we describe the use of a combination of biochemical and biophysical methods to investigate the effect of activator-dependent acetylation on higher order chromatin structure and transcription by RNA polymerase II.

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Source
http://dx.doi.org/10.1007/978-1-61779-477-3_18DOI Listing

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