AI Article Synopsis
- Site-specific recombinases are genetic tools used for manipulating DNA, allowing for the gain, loss, or rearrangement of genetic material in various organisms.
- In Saccharomyces cerevisiae (yeast), these enzymes help study chromosomal elements by turning them into DNA circles, which helps isolate their functions from surrounding genetic influences.
- The chapter covers practical aspects of using site-specific recombination to create these DNA circles in yeast, focusing on the R recombinase's specific applications.
Article Abstract
Site-specific recombinases have been harnessed for a variety of genetic manipulations involving the gain, loss, or rearrangement of genomic DNA in a variety of organisms. The enzymes have been further exploited in the model eukaryote Saccharomyces cerevisiae for mechanistic studies involving chromosomal context. In these cases, a chromosomal element of interest is converted into a DNA circle within living cells, thereby uncoupling the element from neighboring regulatory sequences, obligatory chromosomal events, and other context-dependent effects that could alter or mask intrinsic functions of the element. In this chapter, I discuss general considerations in using site-specific recombination to create DNA circles in yeast and the specific application of the R recombinase.
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| http://dx.doi.org/10.1007/978-1-61779-477-3_7 | DOI Listing |
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