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| http://dx.doi.org/10.1007/978-1-4614-0631-0_11 | DOI Listing |
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a-SiC heteromorphic immersion nanocavities enabling wide-field real-time single-molecule detection.
Biosens Bioelectron
February 2025
School of Biomedical Engineering (Suzhou), Division of Life Sciences and Medicine, University of Science and Technology of China, 230026, Hefei, China; CAS Key Lab of Bio-Medical Diagnostics, Suzhou Institute of Biomedical Engineering and Technology, Chinese Academy of Sciences, 215163, Suzhou, China. Electronic address:
Real-time single-molecule detection via fluorescence exhibits advantages of non-contact and specificity, especially in illustrating the dynamic heterogeneity of living substances. However, wide-field view and signal-to-noise ratio (SNR) are always contradictory in real-time single-molecule detection with fluorescence labels, owing to the limitation of the omnidirectional radiation characteristics of fluorophores. Herein, we propose a nano optical sensing device based on a-SiC heteromorphic immersion nanocavities (aHINCs), enabling wide-field real-time single-molecule imaging without sacrificing SNR.
View Article and Find Full Text PDFDetection of Hepatocellular Carcinoma in an Orthotopic Patient-Derived Xenograft with an Epithelial Cell Adhesion Molecule-Specific Peptide.
Cancers (Basel)
August 2024
Department of Internal Medicine, Division of Gastroenterology, University of Michigan, Ann Arbor, MI 48109, USA.
Article Synopsis
- Hepatocellular carcinoma (HCC) is a significant global health issue, and there’s a need for better detection and treatment methods, like image-guided surgery.
- Researchers used phage display techniques to develop peptides that specifically bind to the EpCAM protein found in HCC, achieving high tumor concentration and stability in the bloodstream.
- The study identified a promising peptide that showed strong binding to HCC cells, effective targeting in animal models, and no signs of toxicity, making it a potential candidate for future cancer diagnostics and therapy.
Quo Vadis Hyperpolarized C MRI?
Z Med Phys
December 2023
Department of Nuclear Medicine, TUM School of Medicine and Health, Klinikum rechts der Isar of Technical University of Munich, 81675 Munich, Germany; Munich Institute of Biomedical Engineering, Technical University of Munich, 85748 Garching, Germany; German Cancer Consortium (DKTK), Partner Site Munich and German Cancer Research Center (DKFZ), Im Neuenheimer Feld 280, Heidelberg 69120, Germany. Electronic address:
Over the last two decades, hyperpolarized C MRI has gained significance in both preclinical and clinical studies, hereby relying on technologies like PHIP-SAH (ParaHydrogen-Induced Polarization-Side Arm Hydrogenation), SABRE (Signal Amplification by Reversible Exchange), and dDNP (dissolution Dynamic Nuclear Polarization), with dDNP being applied in humans. A clinical dDNP polarizer has enabled studies across 24 sites, despite challenges like high cost and slow polarization. Parahydrogen-based techniques like SABRE and PHIP offer faster, more cost-efficient alternatives but require molecule-specific optimization.
View Article and Find Full Text PDFSingle-Molecule Surface-Enhanced Raman Scattering Measurements Enabled by Plasmonic DNA Origami Nanoantennas.
J Vis Exp
July 2023
Institute of Chemistry, University of Potsdam;
Article Synopsis
- - SERS (Surface-enhanced Raman scattering) is a powerful technique that allows for the detection of individual molecules by using high field enhancement, providing detailed spectroscopic information that surpasses traditional methods.
- - The protocol described focuses on using a DNA origami nanoantenna (DONA) combined with atomic force microscopy (AFM) and Raman spectroscopy to achieve single-molecule detection.
- - By using a DNA origami fork structure with gold nanoparticles, the setup creates a gap that enhances SERS signals by up to 10 times, allowing researchers to analyze single molecules more effectively.
HistoSnap: A Novel Software Tool to Extract m/z-Specific Images from Large MSHC Datasets.
Methods Mol Biol
July 2023
ProteoFormiX, Beerse, Belgium.
We describe an informatics tool for comfortable browsing through highly complex, multi-gigabyte mass spectrometry histochemistry (MSHC) datasets, via clever ion-specific image extraction.The package is developed particularly for the untargeted localization/discovery of biomolecules such as endogenous (neuro)secretory peptides on histological sections of biobanked formaldehyde-fixed paraffin-embedded (FFPE) samples straight from tissue banks.Atmospheric pressure-MALDI-Orbitrap MSHC data of sections through human pituitary adenomas in which two well-known human neuropeptides are detected are used as an example to demonstrate the key features of the novel software, named HistoSnap.
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