AI Article Synopsis
- Pea has faced challenges in studying nodulation and mycorrhizae formation due to issues with root transformation and poor growth on agar.
- A new transformation technique has been developed that successfully complements two non-nodulating pea mutants.
- Additionally, methods for rhizobial and fungal inoculation have been introduced to facilitate the study of early nodule development and mycorrhizal root infection, respectively.
Article Abstract
Pea has lagged behind other model legumes in the molecular study of nodulation and mycorrhizae-formation because of the difficulty to transform its roots and its poor growth on agar plates. Here we describe for pea 1) a transformation technique which permits the complementation of two known non-nodulating pea mutants, 2) a rhizobial inoculation method which allows the study of early cellular events giving rise to nodule primordia, and 3) a targeted fungal inoculation method which allows us to study short segments of mycorrhizal roots assured to be infected. These tools are certain to advance our knowledge of pea root symbioses.
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Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3264533 | PMC |
| http://dx.doi.org/10.1186/1746-4811-7-46 | DOI Listing |
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