Objective: Leishmania (Viannia) shawi was characterized only recently, and few studies concerning the immunogenic and protective properties of its antigens have been performed. The present study aimed to evaluate the protective potential of the five antigenic fractions isolated from L. (V.) shawi promastigotes in experimental cutaneous leishmaniasis.
Materials And Methods: Soluble antigen from L. (V.) shawi promastigotes was submitted to reverse phase HPLC to purify F1, F2, F3, F4 and F5 antigens. BALB/c mice were immunized once a week for two consecutive weeks by subcutaneous routes in the rump, using 25 μg protein. After 1 week, groups were challenged in the footpad with L. (V.) shawi promastigotes. After 8 weeks, those same mice were sacrificed and parasite burden as well as the cellular and humoral immune responses were evaluated.
Results: F1 and F5-immunized mice restrained lesion progression and parasite load in the skin. However, only the F1 group was able to control the parasitism in lymph nodes, which was associated with low IL-4 and high IFN-γ production; IgG2a isotype was increased in this group. Immunizations with F2, F3 and F4 antigens did not protect mice.
Conclusion: The capability of antigens to restrain IL-4 levels and increase IFN-γ was associated with protection, such as in immunization using F1 antigen.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1007/s00011-011-0407-5 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
High Selectivity of 8-Hydroxyquinoline on and Species Correlates with a Potent Therapeutic Activity In Vivo.
Pharmaceuticals (Basel)
May 2023
Institute of Biosciences, São Paulo State University (UNESP), Praça Infante Dom Henrique, s/n, São Vicente 11330-900, Brazil.
Leishmaniasis is a neglected disease caused by protozoa of the genus , which causes different clinical manifestations. Drugs currently used in the treatment such as pentavalent antimonial and amphotericin B cause severe side effects in patients, and parasite resistance has been reported. Thus, it is necessary and urgent to characterize new and effective alternative drugs to replace the current chemotherapy of leishmaniasis.
View Article and Find Full Text PDFAmino acid permease 3 (aap3) coding sequence as a target for Leishmania identification and diagnosis of leishmaniases using high resolution melting analysis.
Parasit Vectors
July 2018
Department of Physiology, Institute of Biosciences, University of São Paulo, Rua do Matão Travessa 14 no. 101, São Paulo, SP, 05508900, Brazil.
Background: The leishmaniases comprise a spectrum of clinical manifestations caused by different species of Leishmania. Identification of species is important for diagnosis, treatment and follow-up management. However, there is no gold standard for species identification.
View Article and Find Full Text PDFHigh Resolution Melting Analysis Targeting hsp70 as a Fast and Efficient Method for the Discrimination of Leishmania Species.
PLoS Negl Trop Dis
February 2016
Physiology Department, Biosciences Institute, São Paulo University, São Paulo, São Paulo, Brazil.
Background: Protozoan parasites of the genus Leishmania cause a large spectrum of clinical manifestations known as Leishmaniases. These diseases are increasingly important public health problems in many countries both within and outside endemic regions. Thus, an accurate differential diagnosis is extremely relevant for understanding epidemiological profiles and for the administration of the best therapeutic protocol.
View Article and Find Full Text PDFAntileishmanial activity evaluation of adunchalcone, a new prenylated dihydrochalcone from Piper aduncum L.
Fitoterapia
September 2014
Instituto de Ciências Ambientais, Químicas e Farmacêuticas, Universidade Federal de São Paulo, São Paulo, Brazil. Electronic address:
Bioactivity-guided fractionation of EtOH extract from the leaves of Piper aduncum L. (Piperaceae) afforded a new dihydrochalcone, named adunchalcone. Its structure was elucidated on the basis of their spectroscopic data, primarily NMR and MS.
View Article and Find Full Text PDFIn vitro cytokines profile and ultrastructural changes of microglia and macrophages following interaction with Leishmania.
Parasitology
July 2014
Laboratório de Microscopia Eletrônica,Instituto Evandro Chagas,Av. Almirante Barroso 492, Bairro Marco, 66090-000, Belém, Pará,Brazil.
In the present study, we assessed morphological changes and cytokine production after in vitro interaction with causative agents of American cutaneous leishmaniasis and compared the microglia and macrophage immune responses. Cultures of microglia and macrophages infected with stationary-phase promastigotes of Leishmania (Viannia) shawi, Leishmania (Viannia) braziliensis or Leishmania (Leishmania) amazonensis were evaluated 24, 48 and 72 h after interaction. Macrophages only presented the classical phagocytic process while microglia also displayed large cytoplasmic projections similar to the ruffles described in macropinocytosis.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!