In this paper, a new method of one-pot biosynthesizing of gold nanoparticles (GNPs), using chloroplasts as reductants and stabilizers is reported. The as-prepared GNPs were characterized by ultraviolet visible spectroscopy, transmission electron microscopy, X-ray powder diffraction, and Fourier transform infrared spectroscopy (FTIR). The cytotoxicity of the GNPs was evaluated using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method against gastric mucous cell line GES-1 and gastric cancer cell line MGC-803. Rhodamine 6G as a Raman probe was used for investigating surface-enhanced Raman spectroscopy (SERS) enhancement of GNPs. The transmission electron microscopy results indicated that the GNPs were spherical in structure and almost 20 nm in diameter. Ultraviolet visible spectroscopy exhibited an absorption peak at 545 nm. The GNPs exhibited high crystallinity, with the (111) plane as the predominant orientation, clarified by X-ray powder diffraction. In addition, a potential mechanism was proposed to interpret the formation process of GNPs, mainly based on the analysis of FTIR results. The FTIR spectrum confirmed that the GNPs were carried with N-H groups. Toxicological assays of as-prepared GNPs revealed that the green GNPs were nontoxic. SERS analysis revealed that the GNPs without any treatment could substantially enhance the Raman signals of rhodamine 6G. The Raman enhancement factor was calculated to be nearly 10(10) orders of magnitude. In conclusion, the GNPs with good biocompatibility and excellent SERS effect were successfully synthesized using chloroplasts. These biogenetic GNPs have great potential for ultrasensitive detection of biomarkers in vitro and in vivo based on SERS.
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http://dx.doi.org/10.2147/IJN.S24785 | DOI Listing |
Int J Nanomedicine
December 2024
Department of Obstetrics and Gynecology, Zhongnan Hospital of Wuhan University, Wuhan, Hubei, 430071, People's Republic of China.
Purpose: Fetal nucleated red blood cells (fNRBCs) in the peripheral blood of pregnant women contain comprehensive fetal genetic information, making them an ideal target for non-invasive prenatal diagnosis (NIPD). However, challenges in identifying, enriching, and detecting fNRBCs limit their diagnostic potential.
Methods: To overcome these obstacles, we developed a novel biomimetic chip, replicating the micro-nano structure of red rose petals on polydimethylsiloxane (PDMS).
ACS Appl Mater Interfaces
December 2024
Department of Chemistry, University of Victoria, PO Box 1700 Stn CSC, Victoria, BC V8W 2Y2, Canada.
Gold nanoparticles (GNPs) encapsulated in amphiphilic block copolymers are a promising system for numerous biomedical applications, although critical information on the effects of various preparation variables on the structure and properties of this unique type of nanomaterial is currently missing from the literature. In this research, we synthesized GNPs functionalized with thiol-terminated polycaprolactone (PCL-GNPs) before encapsulating them into poly(ε-caprolactone)--poly(ethylene glycol) (PCL--PEG) micellar nanoparticles via nanoprecipitation to yield GNP-loaded polymeric nanoparticles (GNP-PNPs). We explored the role of different manufacturing variables (water volume, PCL--PEG to PCL-GNP ratio, and PEG block length) on the sizes, morphologies, GNP occupancies, colloidal gold concentrations, and time stability of GNP-PNPs.
View Article and Find Full Text PDFJ Pharm Sci
December 2024
Collaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of Shandong, Key Laboratory of Molecular Pharmacology and Drug Evaluation, Ministry of Education, School of Pharmacy, Yantai University, Yantai, Shandong 264005, PR China.
Glioblastoma (GBM) is a highly aggressive malignant brain tumour which presents a significant challenge due to the limited effectiveness of current surgical and chemotherapeutic approaches. In this study, we have developed TMZ16e and gold nanoparticles coencapsulated thermosensitive liposomes modified with anti-EphA3 (anti-EphA3-TMZ16e-GNPs-TSL) delivered via the intranasal route to achieve photothermal chemotherapy (PCT) for improving the therapeutic effects of GBM. The prepared anti-EphA3-TMZ16e-GNPs-TSL were spherical with a particle size of 173.
View Article and Find Full Text PDFData Brief
December 2024
San Jose State University, 1 Washington Sq., San Jose, CA 95192, United States.
Hybrid epoxy composites are highly considered for low-density applications due to the excellent specific strength and specific stiffness. Enhancements made to the epoxy matrix by addition of nanofillers like carbon nanotubes (CNTs) and graphene (GNPs) have been studied in detail over the course of few decades. These enhancements not only help elevate the material properties of the matrix but also activate different failure mitigating mechanisms in the composite.
View Article and Find Full Text PDFMaterials (Basel)
December 2024
Department of Enterprise Engineering "Mario Lucertini", University of Rome Tor Vergata, 00133 Rome, Italy.
Copper, though highly conductive, requires improved wear resistance and thermal dissipation in applications that involve continuous movement and current-induced vibrations, such as power breakers. Conventional solutions, such as copper-tungsten alloys or lubricant use, face limitations in durability, friction, or environmental impact. This study explores the development of copper-graphene (Cu-GNPs) composite coatings using pulsed electrodeposition to enhance the tribological, thermal, and mechanical properties of circuit breaker components by adopting an industrially scalable technique.
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