Alteration in viability and proliferation activity of mitogen stimulated Jurkat cells.

The aim of our study was the establishment of mechanisms of alterations in viability and mitogen stimulated T lymphocyte proliferation activity. In PHA-stimulated Jurkat cells along with increased proliferation level the reduction of mitochondrial dehydrogenases activity (by 20%) and rising number of cells with low mitochondrial membrane potential (ΔΨm) was revealed. It was concluded that interaction of mitogen (PHA) with T cells receptors (TCR) contributes reduction activity of mitochondrial NADH-dehydrogenase (via phospolipasa C-diacilglicerol (PLC-DAG)-induced PKCs (PKCα, PKCθ) activation pathway). Supression activity of I complex (NADH-dehydrogenase) of mitochondrial respiratory chain and reduction of mitochondrial membrane potencial (ΔΨm) is accompanied with intensification of superoxide radicals production. Superoxide radicals as secondary messengers involve in the induction of T cells viability and proliferative activity regulating genes (CD95L and IL-2) and by this way contribute modification of cell proliferation and apoptosis intensity.