AI Article Synopsis
- Human ADAMTS-18 is a secreted protein that helps in breaking down blood clots, particularly effective with its specific 385-amino acid fragment, AD18C.
- Researchers developed a polyclonal antibody (pAb) against this AD18C fragment by immunizing rabbits, which showed strong binding to both the recombinant and natural forms of the protein.
- The pAb was found to significantly shorten mouse tail bleeding time in a dose-dependent manner, indicating its potential for regulating blood clot stability in clinical settings.
Article Abstract
The human ADAMTS-18, a disintegrin and metalloproteinase with thrombospondin type-1 modules 18, is a secreted Zn-metalloproteinase. The C-terminal 385-amino acid fragment of ADAMTS-18 (AD18C) is highly effective at promoting platelet thrombus dissolution in vivo. Therefore, polyclonal antibody (pAb) against AD18C fragment should be able to keep platelet thrombus stability, which has direct clinical relevance. In this report, pAb against AD18C fragment was generated from rabbit immunized with AD18C recombinant protein (rAD18C). The pAb showed specific binding with rAD18C and natural ADAMTS-18 protein by ELISA and Western blot assay. It shortens the mouse tail bleeding time in a dose-dependent manner. Thus, anti-AD18C pAb contributes to the regulation of platelet thrombus stability.
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| http://dx.doi.org/10.1089/hyb.2011.0055 | DOI Listing |
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