Catalase was covalently immobilized onto florisil via glutaraldehyde (GA) and glutaraldehyde+6-amino hexanoic acid (6-AHA) (as a spacer arm). Immobilizations of catalase onto modified supports were optimized to improve the efficiency of the overall immobilization procedures. The V(max) values of catalase immobilized via glutaraldehyde (CIG) and catalase immobilized via glutaraldehyde+6-amino hexanoic acid (CIG-6-AHA) were about 0.6 and 3.4% of free catalase, respectively. The usage of 6-AHA as a spacer arm caused about 40 folds increase in catalytic efficiency of CIG-6-AHA (8.3 × 10⁵ M⁻¹ s⁻¹) as compared to that of CIG (2.1 × 10⁴ M⁻¹ s⁻¹). CIG and CIG-6-AHA retained 67 and 35% of their initial activities at 5 °C and 71 and 18% of their initial activities, respectively at room temperature at the end of 6 days. Operational stabilities of CIG and CIG-6-AHA were investigated in batch and plug-flow type reactors. The highest total amount of decomposed hydrogen peroxide (TAD-H₂O₂) was determined as 219.5 μmol for CIG-6-AHA in plug-flow type reactor.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.enzmictec.2011.09.002 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Genome reduction improves octanoic acid production in scale down bioreactors.
Microb Biotechnol
November 2024
Department of Chemical and Biological Engineering, University of Wisconsin-Madison, Madison, Wisconsin, USA.
Microorganisms in large-scale bioreactors are exposed to heterogeneous environmental conditions due to physical mixing constraints. Nutritional gradients can lead to transient expression of energetically wasteful stress responses and as a result, can reduce the titres, rates and yields of a bioprocess at larger scales. To what extent these process parameters are impacted is often unknown and therefore bioprocess scale-up comes with major risk.
View Article and Find Full Text PDFHigh-solid digestion - A comparison of completely stirred and plug-flow reactor systems.
Waste Manag
December 2024
Department of Molecular Sciences, Swedish University of Agricultural Sciences, Uppsala, Sweden; Biogas Solutions Research Center, Linköping, Sweden. Electronic address:
High-solid digestion (HSD) for biogas production is a resource-efficient and sustainable method to treat organic wastes with high total solids content and obtain renewable energy and an organic fertiliser, using a lower dilution rate than in the more common wet digestion process. This study examined the effect of reactor type on the performance of an HSD process, comparing plug-flow (PFR) type reactors developed for continuous HSD processes, and completely stirred-tank reactors (CSTRs) commonly used for wet digestion. The HSD process was operated in thermophilic conditions (52 °C), with a mixture of household waste, garden waste and agricultural residues (total solids content 27-28 %).
View Article and Find Full Text PDFRetrofittable plug-flow reactor for in situ high-temperature vibrating sample magnetometry with well-controlled gas atmospheres.
Rev Sci Instrum
June 2023
Department of Physics, Technical University of Denmark, DK-2800 Kgs. Lyngby, Denmark.
We have developed an in situ sample-holder-akin to a quartz-based plug-flow reactor-for vibrating sample magnetometry (VSM) in gas-controlled environments at ambient pressure and temperatures up to ∼1000 °C. The holder matches onto a specific type of vibrating sample magnetometer (Lake Shore model 7404-S), but the principles are applicable to other types of VSM. The holder has been tested on powder samples of Co particles on a MgAl2O4 support in both reducing and oxidizing atmospheres.
View Article and Find Full Text PDFEsterification of Acetic Acid by Flow-Type Membrane Reactor with AEI Zeolite Membrane.
Membranes (Basel)
January 2023
Department of Applied Chemistry, Waseda University, 513 Wasedatsurumaki-cho, Shinjuku-ku, Tokyo 162-0041, Japan.
AEI-type zeolite membrane for dehydration was prepared, and a flow-type membrane reactor for the esterification of acetic acid and ethanol by AEI membrane was developed. A synthesized AEI membrane had suitable molecular sieving property for gas separation (H/-butane and CO/CH) and pervaporation (HO/acetic acid). AEI membrane showed HO permeance of 6.
View Article and Find Full Text PDFComputational fluid dynamics simulation improves the design and characterization of a plug-flow-type scale-down reactor for microbial cultivation processes.
Biotechnol J
January 2023
Department of Biotechnology, Institute of Bioprocess Science and Engineering, University of Natural Resources and Life Sciences, Vienna, Austria.
The scale-up of bioprocesses remains one of the major obstacles in the biotechnology industry. Scale-down bioreactors have been identified as valuable tools to investigate the heterogeneities observed in large-scale tanks at the laboratory scale. Additionally, computational fluid dynamics (CFD) simulations can be used to gain information about fluid flow in tanks used for production.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!