AI Article Synopsis
- A bacterium named Janibacter sp. TYM3221 was isolated and studied for its ability to degrade the pesticide 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE), with a focus on understanding the enzyme systems necessary for this process.
- Analysis revealed that TYM3221 converts DDE into five metabolites, including a key product linked to its degradation pathway—4-chlorobenzoic acid, which indicates a specific chemical transformation process.
- A specific gene cluster (bphAaAbAcAd) responsible for a biphenyl-degrading enzyme was identified, and when this gene was inactivated, the mutant strain lost its ability to degrade both
Article Abstract
Bacterial degradation of 1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene (DDE) has been previously reported, however, its degradation enzyme system has not been characterized. In this study, a DDE-degrading bacterium, Janibacter sp. TYM3221, was isolated and characterized. Transformation of DDE was demonstrated by TYM3211 resting cells grown in LB in the presence and absence of biphenyl. Gas chromatography-mass spectrometry analysis revealed five metabolites of DDE containing a meta-ring cleavage product and 4-chlorobenzoic acid, suggesting that TYM3221 degrades DDE to 4-chlorobenzoic acid via a meta-ring cleavage product. A gene cluster, bphAaAbAcAd, which codes for biphenyl dioxygenase subunits, was cloned from TYM3221. A mutant strain with a bphAa-gene inactivation did not grow on biphenyl, and showed no DDE degradation activity. These results indicate that in strain TYM3221, the bphAa-coded biphenyl dioxygenase is involved not only in the metabolism of biphenyl but also in the degradation of DDE.
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| http://dx.doi.org/10.1016/j.enzmictec.2011.06.017 | DOI Listing |
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