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Thy28 partially prevents apoptosis induction following engagement of membrane immunoglobulin in WEHI-231 B lymphoma cells. | LitMetric

AI Article Synopsis

  • Thy28 protein is found in various organisms, including plants and mammals, and is notably expressed in testis, liver, and immune cells like lymphocytes.
  • The study investigates the role of Thy28 in affecting apoptosis in WEHI-231 B lymphoma cells after activation of membrane immunoglobulin (mIg).
  • Results show that cells overexpressing Thy28 are resistant to apoptosis induced by mIg, maintaining mitochondrial integrity and altering signaling pathways, which is linked to an increase in G1 phase and upregulation of p27(Kip1).

Article Abstract

Thy28 protein is conserved among plants, bacteria, and mammalian cells. Nuclear Thy28 protein is substantially expressed in testis, liver, and immune cells such as lymphocytes. Lymphocyte apoptosis plays a crucial role in homeostasis and formation of a diverse lymphocyte repertoire. In this study, we examined whether Thy28 affects induction of apoptosis in WEHI-231 B lymphoma cells following engagement of membrane immunoglobulin (mIg). Once they were established, the Thy28-overexpressing WEHI-231 cells showed similar expression levels of IgM and class I major histocompatibility complex (MHC) molecule compared with controls. The Thy28-overexpressing cells were considerably resistant to loss of mitochondrial membrane potential (ΔΨm), caspase-3 activation, and increase in annexin-positive cells upon mIg engagement. These changes were concomitant with an increase in G1 phase associated with upregulation of p27(Kip1). The anti-IgM-induced sustained activation of c-Jun N-terminal kinase (JNK), which was associated with late-phase hydrogen peroxide (H(2)O(2)) production, was partially reduced in the Thy28-expressing cells relative to controls. Taken together, the data suggest that in WEHI-231 B lymphoma cells, Thy28 regulates mIg-mediated apoptotic events through the JNK-H(2)O(2) activation pathway, concomitant with an accumulation of cells in G1 phase associated with upregulation of p27(Kip1) in WEHI-231 B lymphoma cells.

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Source
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6275998PMC
http://dx.doi.org/10.2478/s11658-011-0034-8DOI Listing

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