Matrix-based yeast two-hybrid screens are an alternative to library-based screens. Recent improvements of matrix screens (also called array screens), use various pooling strategies as well as novel vectors that increase its efficiency while decreasing the false-negative rate, thus increasing reliability. In this chapter, we describe a screening strategy that systematically combines N- and C-terminal fusion proteins using a recently developed vector system.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7120008 | PMC |
| http://dx.doi.org/10.1007/978-1-61779-424-7_21 | DOI Listing |
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