Transfection of osteoclasts and osteoclast precursors.

Methods Mol Biol

Musculoskeletal Research Programme, Division of Applied Medicine, Institute of Medical Sciences, University of Aberdeen, Foresterhill, Aberdeen, UK.

Published: March 2012

Osteoclasts and their precursors have traditionally been considered difficult cells to transfect using standard approaches. Here, we describe several methods for transfection of mature osteoclasts and their precursors using the Amaxa™ Nucleofector system, lentiviruses, and adenoviruses.

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http://dx.doi.org/10.1007/978-1-61779-415-5_14DOI Listing

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