Objective: To establish a method based on molecular beacon real-time PCR for detecting single nucleotide polymorphisms (SNP) in codon 72 of scar-related p53 gene.
Methods: Two fluorescence-labeled molecular beacon probes were synthesized targeting CCC/CGC SNP of p53 codon 72. The genomic DNA was extracted from the peripheral blood of 28 patients with keloid, and the CCC/CGC SNP of P53 gene codon 72 were assayed with molecular beacon real-time PCR. The results of SNP typing were compared with the results of reverse dot hybridization and confirmed by direct DNA sequencing.
Results: The goodness of fit of this method was 100% in comparison with direct DNA sequencing, higher than that of reverse dot hybridization.
Conclusion: Molecular beacon real-time PCR is suitable for rapid clinical detection of SNPs in p53 gene.
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