An isomelezitose synthase was redesigned out of the sucrose isomerase from Protaminobacter rubrum for the synthesis of isomelezitose (6-O(F)-glucosylsucrose), a potential nutraceutical. The variants F297A, F297P, R333K, F321A_F319A and E428D catalyze the formation of isomelezitose in up to 70 % yield.
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Simple, fast, and efficient process for producing and purifying trehalulose.
Food Chem
June 2013
State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangxi University, 100 Daxue Road, Nanning, Guangxi 530004, China.
A new property of recombinant trehalose synthase (GTase) from Thermus thermophilus HB-8 (ATCC 27634) was found and described in this study. GTase can act on sucrose and catalyze trehalulose formation without isomaltose, isomaltulose, or isomelezitose, releasing small amounts of glucose and fructose as byproducts. Maximum trehalulose yield (approximately 81%) was obtained at an optimum temperature of 65°C and was independent of substrate concentration.
View Article and Find Full Text PDFMechanism-oriented redesign of an isomaltulose synthase to an isomelezitose synthase by site-directed mutagenesis.
Chembiochem
January 2012
Institute of Organic Chemistry, Julius-Maximilians University Würzburg, Würzburg, Germany.
An isomelezitose synthase was redesigned out of the sucrose isomerase from Protaminobacter rubrum for the synthesis of isomelezitose (6-O(F)-glucosylsucrose), a potential nutraceutical. The variants F297A, F297P, R333K, F321A_F319A and E428D catalyze the formation of isomelezitose in up to 70 % yield.
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