Streptomyces sp. strain Wigar10 was isolated from a surface-sterilized garlic bulb (Allium sativum var. Purple Stripe). Its genome encodes several novel secondary metabolite biosynthetic gene clusters and provides a genetic basis for further investigation of this strain's chemical biology and potential for interaction with its garlic host.
Download full-text PDF |
Source |
|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3232846 | PMC |
| http://dx.doi.org/10.1128/JB.06257-11 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Coordinated regulation of two LacI family regulators, GvmR and GvmR2, on guvermectin production in .
Synth Syst Biotechnol
November 2024
Key Laboratory of Agricultural Microbiology of Heilongjiang Province, Northeast Agricultural University, Harbin, 150030, China.
Guvermectin, a purine nucleoside natural product produced by the genus S, has recently been registered as a new biopesticide to boost rice yield. Despite its economic and agricultural significance, the regulatory mechanisms of guvermectin biosynthesis remain essentially unknown, hindering industrial production and widespread agricultural application. Here, we examined the roles of two LacI family regulators, and , located within and adjacent to the guvermectin biosynthesis cluster, respectively, in guvermectin production in NEAU6.
View Article and Find Full Text PDFCurrent Approaches for Genetic Manipulation of spp.-Key Bacteria for Biotechnology and Environment.
BioTech (Basel)
January 2025
Valent BioSciences, Biorational Research Center, 1910 Innovation Way, Suite 100, Libertyville, IL 60048, USA.
Organisms from the genus feature actinobacteria with complex developmental cycles and a great ability to produce a variety of natural products. These soil bacteria produce more than 2/3 of antibiotics used in medicine, and a large variety of bioactive compounds for industrial, medical and agricultural use. Although spp.
View Article and Find Full Text PDFIdentification of a novel butenolide signal system to regulate high production of tylosin in Streptomyces fradiae.
Appl Microbiol Biotechnol
January 2025
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, 100101, China.
Identifying hormone-like quorum sensing (QS) molecules in streptomycetes is challenging due to low production levels but is essential for understanding secondary metabolite biosynthesis and morphological differentiation. This work reports the discovery of a novel γ-butenolide-type signaling molecule (SFB1) via overexpressing its biosynthetic gene (orf18) in Streptomyces fradiae. SFB1 was found to be essential for production of tylosin through dissociating the binding of its receptor TylP (a transcriptional repressor) to target genes, thus activating the expression of tylosin biosynthetic gene cluster (tyl).
View Article and Find Full Text PDFOptimizing genome editing efficiency in via a CRISPR/Cas9n-mediated editing system.
Appl Environ Microbiol
January 2025
State Key Laboratory of Biocatalysis and Enzyme Engineering, Environmental Microbial Technology Center of Hubei Province, College of Life Sciences, Hubei University, Wuhan, China.
is an important bioresource to produce various antibacterial natural products, however, the time-consuming and labor-intensive genome editing toolkits hindered the construction and application of engineered strains, and this study aimed to establish an efficient CRISPR/Cas9n genome editing system in . Initially, the CRISPR/Cas9-mediated editing tool was employed to replace those awkward genome editing tools that relied on homologous recombination, while the off-target Cas9 exhibited high toxicity to Sf01. Therefore, the nickase mutation D10A, high-fidelity mutations including N497A, R661A, Q695A, and Q926A, and thiostrepton-induced promotor P were incorporated into the Cas9 expression cassette, which reduced its toxicity.
View Article and Find Full Text PDFOptimizing the production and efficacy of antimicrobial bioactive compounds from in combating multi-drug-resistant pathogens.
Front Cell Infect Microbiol
January 2025
Mkelly Biotech Pvt Ltd., Mohali, Punjab, India.
Background: The rise of antibiotic-resistant pathogens has intensified the search for novel antimicrobial agents. This study aimed to isolate from local soil samples and evaluate its antimicrobial properties, along with optimizing the production of bioactive compounds.
Methods: Soil samples were collected from local regions, processed, and analysed for Streptomyces strains isolation using morphological characteristics and molecular identification through 16S rRNA gene PCR assay.
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!