A method for generation of highly specific miniantibodies within the phage particle has been developed, and used to produce antibodies against Staphylococcus enterotoxin type C1. Under successive panning of the non-immune phage miniantibody (scFv) library with enterotoxins SE (types A, B, C1, D, E, G, and I) adsorbed on the plate surface, we generated 11 individual phage clones to Staphylococcus enterotoxin type C1. Five of them interacted specifically only with SEC1 and had no cross-reactions with the other enterotoxins.
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|---|---|
| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3242837 | PMC |
| http://dx.doi.org/10.4161/mabs.3.6.18089 | DOI Listing |
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