AI Article Synopsis
- - The study investigates the impact of ionizing radiation on cancer cells, particularly whether it boosts their ability to migrate and invade through a process called epithelial-mesenchymal transition (EMT) driven by TGF-β signaling.
- - Cancer cells from six different origins were exposed to gamma radiation, leading to observable changes like decreased epithelial markers and increased mesenchymal markers, which in turn enhanced their migratory and invasive properties.
- - The findings suggest that TGF-β is crucial in mediating the effects of ionizing radiation on cancer cell behavior, with potential for reversing these effects through the use of specific inhibitors.
Article Abstract
Purpose: To examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT).
Methods And Materials: Six cancer cell lines originating from different human organs were irradiated by 60Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542.
Results: After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling.
Conclusions: These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.
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| http://dx.doi.org/10.1016/j.ijrobp.2011.06.1956 | DOI Listing |
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