AI Article Synopsis
- Multi-dimensional particle tracking is crucial for studying dynamic processes in both living and synthetic soft matter systems, and this paper introduces a novel method for tracking micron-sized beads in three dimensions.
- The new approach uses an acousto-optic deflector and quadrant photo-diodes to achieve faster tracking at tens of kHz with nanometer precision, enabling individual calibration of multiple traps in just a few seconds.
- The study includes experiments on hydrodynamic coupling and diffusion anomalies using a 3 × 3 arrangement of trapped beads, with potential for tracking more in various 2D configurations.
Article Abstract
Multi-dimensional, correlated particle tracking is a key technology to reveal dynamic processes in living and synthetic soft matter systems. In this paper we present a new method for tracking micron-sized beads in parallel and in all three dimensions - faster and more precise than existing techniques. Using an acousto-optic deflector and two quadrant-photo-diodes, we can track numerous optically trapped beads at up to tens of kHz with a precision of a few nanometers by back-focal plane interferometry. By time-multiplexing the laser focus, we can calibrate individually all traps and all tracking signals in a few seconds and in 3D. We show 3D histograms and calibration constants for nine beads in a quadratic arrangement, although trapping and tracking is easily possible for more beads also in arbitrary 2D arrangements. As an application, we investigate the hydrodynamic coupling and diffusion anomalies of spheres trapped in a 3 × 3 arrangement.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1364/OE.19.021627 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Want AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!