Optimising DNA extraction from clinical samples for Burkholderia pseudomallei Type III secretion system real-time PCR in suspected melioidosis patients confirmed that urine and sputum are useful diagnostic samples. Direct testing on blood remains problematic; testing DNA extracted from plasma was superior to DNA from whole blood or buffy coat.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3249147 | PMC |
| http://dx.doi.org/10.1016/j.mimet.2011.10.023 | DOI Listing |
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