Expression of the lipase gene from Staphylococcus hyicus in Lactobacillus curvatus Lc2-c.

An electroporation based transformation system was developed for Lactobacillus curvatus Lc2-c, a plasmid cured potential raw sausage starter. It was transformed with plasmid pLipPS1, a staphylococcal vector plasmid containing the lipase gene from Staphylococcus hyicus. Transformants exhibited high lipase activity, whereas the wild type strain was lipase negative. The production and release into the medium of a heterologous lipase by a Lactobacillus could be shown for the first time.