AI Article Synopsis
- C836 is a monoclonal antibody that specifically targets human interleukin IL-13, developed through mouse immunization.
- The crystal structure of C836 Fab was resolved at 2.5 Å resolution and was analyzed alongside a previously determined IL-13-bound structure.
- The comparison reveals a significant "induced-fit" mechanism, marked by a large rigid-body rotation of its variable domains during the binding process.
Article Abstract
C836 is a neutralizing monoclonal antibody to human interleukin IL-13 generated by mouse immunization. The crystal structure of the C836 Fab was determined at 2.5 Å resolution and compared with the IL-13-bound form determined previously. This comparison indicates an induced-fit mechanism of antigen recognition through rigid-body rotation of the V(L) and V(H) domains. The magnitude of this rearrangement is one of the largest observed for antibody-protein interactions.
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| http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3212354 | PMC |
| http://dx.doi.org/10.1107/S1744309111027746 | DOI Listing |
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