We describe a method for expanding the linear dynamic range for multiple reaction monitoring (MRM) in quantitative liquid chromatography/tandem mass spectrometry (LC-MS/MS) using additional transitions for isotopologues. In addition to the regular transition for the highest possible sensitivity, a transition corresponding to the less abundant isotopologue ions was utilized. This decreases saturation at the ion detector; the sensitivity reduction increases the upper dynamic limit. We demonstrated this for a rat plasma assay for a candidate flavor compound; the linear dynamic range increased by an order of magnitude from 3 to 6,000 ng/mL with the regular MRM alone to 3-60,000 ng/mL using additionally the isotopologue transition.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.talanta.2011.09.063 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Elevated triglyceride glucose index is associated with advanced cardiovascular kidney metabolic syndrome.
Sci Rep
December 2024
Department of Cardiology, The First Affiliated Hospital, Guangzhou Medical University, Guangzhou, Guangdong, 510120, China.
The cardiovascular kidney metabolic (CKM) syndrome is a dynamic geriatric condition that has received limited research attention regarding its potential associations with the triglyceride glucose (TyG) index. This study aims to explore the potential association between the TyG index and advanced CKM syndrome. Data for this cross-sectional study were obtained from the National Health and Nutrition Examination Survey (NHANES) conducted between 2011 and 2018.
View Article and Find Full Text PDFInterspecific competition and survival pressures in endangered Barringtonia racemosa populations of Mainland China.
Sci Rep
December 2024
Guangxi Subtropical Crops Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530001, China.
This study evaluates the growth, survival pressures, and community dynamics of Barringtonia racemosa (L.) Spreng. populations in Jiulong Mountain and Suixi County, Guangdong Province.
View Article and Find Full Text PDFThe relationship between residual insulin secretion and subclinical cardiovascular risk indices in young adults with type 1 diabetes.
J Diabetes Complications
December 2024
National and Kapodistrian University of Athens First Department of Propaedeutic and Internal Medicine, Laiko General Hospital Athens, Attiki, Greece.
Background: Patients with type 1 diabetes (DM1), even in the setting of adequate glycaemic control, have an excess risk for developing cardiovascular disease. Residual insulin secretion (RIS), measured by detectable C-peptide levels in patients with DM1, might protect against diabetes-related complications. This study aimed to examine the relationship between residual insulin secretion and prognostic markers of cardiovascular complications in patients with DM1.
View Article and Find Full Text PDFMulti-scale temporal and spatial variations of soil heat flux under varying riparian forests: From a day to a year.
J Environ Manage
December 2024
School of Design, Shanghai Jiao Tong University, Shanghai, 200240, China. Electronic address:
This study delves into the multi-scale temporal and spatial variations of soil heat flux (G) within riparian zones and its correlation with net radiation (Rn) across six riparian woodlands in Shanghai, each characterized by distinct vegetation types. The objective is to assess the complex interrelations between G and Rn, and how these relationships are influenced by varying vegetation and seasons. Over the course of a year, data on G and Rn is collected to investigate their dynamics.
View Article and Find Full Text PDFDigital phenotyping from heart rate dynamics: Identification of zero-poles models with data-driven evolutionary learning.
Comput Biol Med
December 2024
Complex Systems Laboratory, University Politehnica of Bucharest, Bucharest, Romania; Centre for Elderly and Nursing Home Medicine, University of Bergen, Bergen, Norway; Neuro-SysMed Center, University of Bergen, Bergen, Norway. Electronic address:
Heart rate response to physical activity is widely investigated in clinical and training practice, as it provides information on a person's physical state. For emerging digital phenotyping approaches, there is a need for individualized model estimation. In this study, we propose a zero-poles model and a data-driven evolutionary learning method for identification.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!