Background/aims: Different models of reconstructed skin are available, either to provide skin wound healing when this process is deficient, or to be used as an in vitro model. Nevertheless, few studies have focused on the mechanical properties of skin equivalent. Indeed, human skin is naturally under tension. Taking into account these features, the purpose of this work was to obtain a cellularized dermal equivalent (CDE), composed of collagen and dermal fibroblasts.
Methods: To counteract the natural retraction of CDE and to maintain it under tension, different biomaterials were tested. Selection criteria were biocompatibility, bioadhesion properties, ability to induce differentiation of fibroblasts into myofibroblasts and mechanical characterization, considering that of skin in vivo. These assays led to the selection of honeycomb of polyester. CDE constructed on this biomaterial was further characterized mechanically using tensile tests.
Results: The results showed that mechanical features of the obtained dermal equivalent, including myofibroblasts, were similar to skin in vivo.
Conclusion: The original model of dermal equivalent presented herein may be a useful tool for clinical use and as an in vitro model for toxicological/pharmacological research.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1111/j.1600-0846.2011.00564.x | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Distribution of carcinogenic polycyclic aromatic hydrocarbons in urban soil across major cities of Bihar, India: seasonal variation, source apportionment, and health risk assessment.
Environ Monit Assess
December 2024
Department of Environmental Science, Central University of South Bihar, SH-7, Gaya Panchanpur, Post-Fatehpur, P.S-Tekari, District-Gaya, 824236, Bihar, India.
This study investigates the distribution of sixteen priority polycyclic aromatic hydrocarbons (PAHs), various sources of PAHs, and their probable cancer-causing risks in the soil samples collected from urban cities in Bihar, India. During the winter season, the ∑ PAH concentration was dominant and ranged from 979.36 to 5149.
View Article and Find Full Text PDFIncorporating immune cell surrogates into a full-thickness tissue equivalent of human skin to characterize dendritic cell activation.
Sci Rep
December 2024
Institute of Pharmaceutical Biology and Biotechnology, Heinrich Heine University Düsseldorf, Universitätsstr.1, 40225, Düsseldorf, Germany.
Article Synopsis
- Research on dendritic cell (DC) activation has mostly relied on animal models, highlighting the need for human-based in vitro models due to differences in DC types across species.
- Scientists have created a full-thickness human skin tissue model with Langerhans cell (LC) and dermal dendritic cell (DDC) surrogates from human leukemia cell lines to study their activation.
- When exposed to nickel sulfate or DNCB, the model showed significant increases in CD1a positive cells, indicating that these treatments trigger a response leading to DC migration and activation within a short time frame.
Regulation of Tumor Growth and Invasion in Oral Squamous Cell Carcinoma by the Tumor Microenvironment Through the Enhancement of IGFBP-3 Expression.
Anticancer Res
December 2024
Department of Dental Hygiene, College of Health Science, Eulji University, Seongnam, Republic of Korea
Background/aim: Accumulated evidence indicates that interactions among various stromal cells within the tumor microenvironment (TME) significantly influence cancer progression. Oral cancers not diagnosed at early stages are associated with low five-year survival rates, highlighting the need for substantial improvements in patient outcomes. Understanding the interactions between cancer cells and the tumor microenvironment is crucial for identifying methods and developing treatment strategies that more effectively inhibit tumor progression and metastasis.
View Article and Find Full Text PDFEffects of pyrolysis conditions on sewage sludge-biochar properties and potential risks based on PAH contents.
Environ Res
November 2024
Southern Federal University, Rostov-on-Don, Russian Federation. Electronic address:
Pyrolysis of sewage sludge can significantly reduce industrial waste while producing high-value biochar for soil improvement. This study aimed to evaluate the quality and safety of biochar from sewage sludge under different pyrolysis conditions. Optimal carbonization conditions (700 °C, 60 min, 5 °C/min) were identified by analyzing the physicochemical properties, elemental composition, structural characteristics, and the specific surface area of biochar.
View Article and Find Full Text PDFTailored 3D Agarose-Well Integrated with Human Skin Equivalents for Enhanced Skin Penetration Assessment.
Gels
October 2024
Department of Chemical Engineering, Soongsil University, Seoul 06978, Republic of Korea.
We developed a tailored 3D Agarose-well system integrated with reconstructed human skin equivalents to enhance skin penetration assessments. This system addresses common limitations in traditional trans-well reconstructions, such as dermal layer contraction and limited lateral diffusion, by entangling collagen fibrils within the Agarose-well. We evaluated the penetration behavior of three peptides, with and without skin-penetrating peptide (SPP) sequences, alongside adenosine, a known anti-wrinkle agent.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!