Aim: We report an in vitro technique to establish alignment of collagen fibres and cells within a three-dimensional tissue equivalent that mimics the natural periodontal ligament (PDL) using a novel custom-designed bioreactor.
Material And Methods: Shear stress was applied to the tissue equivalent prepared with collagen solution and seeded with human PDL cells. Stress-strain dynamics and the alignment of collagen fibres and PDL cells in tissue equivalents were analysed.
Results: Shear stress aligned collagen fibres and PDL cells in a direction parallel to the principle strain vector. PDL cells and Collagen fibres aligned in strained tissue equivalents with higher uniformity than in unstrained tissue equivalents.
Conclusions: The cell and fibre alignment of the engineered PDL was precisely guided by mechanical shear stress along the direction of principal strain vector using a custom-designed bioreactor, suggesting that the enhanced functional property of engineered PDL constructs could be achieved with this technique.
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| http://dx.doi.org/10.1111/j.1600-051X.2011.01790.x | DOI Listing |
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