We applied high-pressure liquid chromatography to assess the decomposition of the oxidized form of vitamin C, dehydro-L-ascorbic acid. We selected experimental conditions that might represent a wide variety of clinical and research procedures. Decay of dehydro-L-ascorbic acid proceeded much more rapidly at high pH (7-8) than at low pH (3-5) and was more rapid at 37 or 45 degrees C than at 0 or 23 degrees C. When evaluated at pH 6.6, the percent decay was somewhat more rapid from an initial concentration of 1000 mumol/L than at 5-10 mumol/L. The analytical procedure (HPLC) provided useful information about the rate of decay under various conditions. This may facilitate future biological and clinical studies that require a distinction between the oxidized and reduced forms of vitamin C.
Download full-text PDF |
Source |
|---|
Publication Analysis
Top Keywords
Similar Publications
A novel mıcrobıal bıosensor system based on C. tropicalis yeast cells for selectıve determınatıon of L-Ascorbıc acid.
Bioelectrochemistry
April 2020
Department of Biochemistry, Faculty of Science, Ege University, 35100 Bornova, Izmir, Turkey.
In this study, a novel microbial biosensor was developed for the selective determination of L-Ascorbic acid. In the construction of the microbial biosensor, lyophilized Candida tropicalis yeast cells were immobilized with o-aminophenol by forming a film layer on a platinum electrode surface using electropolymerization. L-Ascorbic acid was quantified on the basis of both amperometric and differential pulse voltammetry (DPV) methods using the biosensor.
View Article and Find Full Text PDFThe oxidation of dehydroascorbic acid and 2,3-diketogulonate by distinct reactive oxygen species.
Biochem J
November 2018
The Edinburgh Cell Wall Group, Institute of Molecular Plant Sciences, The University of Edinburgh, The King's Buildings, Edinburgh EH9 3BF, U.K.
l-Ascorbate, dehydro-l-ascorbic acid (DHA), and 2,3-diketo-l-gulonate (DKG) can all quench reactive oxygen species (ROS) in plants and animals. The vitamin C oxidation products thereby formed are investigated here. DHA and DKG were incubated aerobically at pH 4.
View Article and Find Full Text PDFPotassium (1R,4R,5S,8S)-4,5,8-trihy-droxy-3-oxo-2,6-dioxabicyclo-[3.3.0]octane-4-sulfonate dihydrate.
Acta Crystallogr Sect E Struct Rep Online
January 2013
School of Chemistry, University of East Anglia, Norwich NR4 7TJ, England.
The title salt, K(+)·C6H7O9S(-)·2H2O, formed by reaction of dehydro-l-ascorbic acid with potassium hydrogen sulfite in water, crystallizes as colourless plates. The potassium ion is coordinated by eight O atoms arising from hy-droxy or sulfonate groups. The sulfonate group is bonded to the C atom neighbouring that of the lactone carbonyl group.
View Article and Find Full Text PDFThermal decomposition of vitamin C: An evolved gas analysis-ion attachment mass spectrometry study.
Food Chem
November 2011
Department of Chemistry, Faculty of Sciences and Engineering, Meisei University, Hodokubo 2-1-1, Hino, Tokyo 191-8506, Japan. Electronic address:
Evolved gas analysis-ion attachment mass spectrometry (EGA-IAMS) was utilised to study the real-time non-isothermal decomposition of vitamin C. Dehydro-l-ascorbic acid, which has until this study been undetectable from the solid phase degradation of vitamin C, was observed as a decomposition product. While it is an important compound because it possesses some biological activity, dehydro-l-ascorbic acid is difficult to measure due to its chemical instability.
View Article and Find Full Text PDFDevelopment of a quantitative method for the analysis of total L-ascorbic acid in foods by high-performance liquid chromatography.
J Chromatogr A
June 2007
Department of Internal Medicine, Section of Applied Biochemistry and Nutritional Sciences, University of Perugia, Via San Costanzo, 06126 - Perugia, Italy.
A new method has been developed for the determination of total vitamin C in foods. The method requires less time than the traditional methodologies and uses a radical oxidation of L-ascorbic acid (AA) to obtain dehydro-L-ascorbic acid (DHAA) by means of a peroxyl radical generated in situ by thermal decomposition of an azo-compound, 2,2'-azobis(2-amidinopropane) dihydrochloride (AAPH). The dehydro-L-ascorbic acid is condensed with benzene-1,2-diamine (o-phenylenediamine, OPDA) to form its highly fluorescent quinoxaline derivative, 3-(1,2-dihydroxyethyl)furo[3,4-b]quinoxaline-1-one (DFQ), which is then separated on a C(18) column eluted with a mobile phase of 80 mM phosphate buffer and methanol at pH=7.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!