In this study, the PCR-based DNA strip assay GenoType BC for the identification of bacteria and the resistance genes mecA, vanA, vanB, vanC1, and vanC2/3 directly from positive BacTAlert blood culture bottles was evaluated in a multicenter study. Of a total of 511 positive blood cultures, correct identification percentages for Gram-negative bacteria, Gram-positive bacteria, and the mecA gene were 96.1%, 89.9%, and 92.9%, respectively. Results were available 4 h after growth detection.
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http://dx.doi.org/10.1128/JCM.06086-11 | DOI Listing |
Transfusion
January 2025
Japanese Red Cross Society, Central Blood Institute, Tokyo, Japan.
Background: Reports of cases of bacterial infection due to transfusion of red blood cell (RBC) components (RBC-TTBI) are relatively rare. Hence, the possibility of undetectable bacterial contamination in RBCs, especially by psychrotrophic bacteria, must be clarified.
Study Design And Methods: We assessed nine psychrotrophic bacterial species, including those implicated in bacteremia or RBC-TTBIs.
J Med Microbiol
January 2025
Division of Infection and Global Health, School of Medicine, University of St Andrews, St Andrews, UK.
Bloodstream infections (BSIs) are one of the most serious infections investigated by microbiologists. However, the time to detect a BSI fails to meet the rapidity required to inform clinical decisions in real time. Blood culture (BC) is considered the gold standard for diagnosing bloodstream infections.
View Article and Find Full Text PDFIran J Microbiol
December 2024
Department of Microbiology, Sher-i-Kashmir Institute of Medical Sciences, Jammu and Kashmir, India.
Background And Objectives: The incidence of multidrug-resistant, Gram-negative organisms, isolated as the etiological agents of infections is ascending. The advent of novel antibiotics poses significant challenges, necessitating the optimization and utilization of extant antimicrobial agents. Cefoperazone, a third-generation cephalosporin and β-lactam antimicrobial, when combined with sulbactam, an irreversible β-lactamase inhibitor, mitigates the vulnerability of cefoperazone to β-lactamase-producing organisms.
View Article and Find Full Text PDFAccess Microbiol
September 2024
Medical Affairs and Innovation, Canadian Blood Services, Ottawa, Ontario, Canada.
has been involved in transfusion-transmitted fatalities associated with platelet concentrates (PCs) due to its heightened pathogenicity enhanced by genome-encoded virulence and antibiotic resistance genes. This may be facilitated by mobile genetic elements (MGEs) that can cause rearrangements. Several factors contribute to virulence, including the type VII secretion system (T7SS), composed of six core genes conserved across strains.
View Article and Find Full Text PDFZh Vopr Neirokhir Im N N Burdenko
December 2024
Burdenko Neurosurgical Center, Moscow, Russia.
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