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Objectives: The extended-spectrum β-lactamase (ESBL) PER-1 initially disseminated among Pseudomonas aeruginosa strains in Turkey. Despite reports from other European countries, such strains have not been detected in Greece until now. We describe the first bla(PER-1)-positive P. aeruginosa isolates from Greece and their genetic environment.
Methods: From January 2008 to December 2009, 287 consecutive non-duplicate P. aeruginosa isolates with reduced susceptibility or resistance to ceftazidime (MIC >8 mg/L) were screened for ESBL production with a modified boronic acid-based double-disc synergy test. Phenotypically ESBL-positive isolates were subjected to agar dilution, PFGE and multilocus sequence typing (MLST). Broad-spectrum bla genes were identified by PCR and sequencing. Plasmid analysis and conjugation experiments were performed. The location of the bla(PER-1) gene was detected by Southern blotting and its genetic environment was characterized using inverse PCR.
Results: Five isolates were phenotypically positive for ESBL production, exhibited resistance to cefepime, ceftazidime, aztreonam and meropenem, and carried the bla(PER-1) gene. MLST showed that they belonged to sequence type (ST) 235, which belongs to the international clonal complex 11. Four isolates had the same PFGE pattern. Southern blotting revealed the chromosomal location of the bla(PER-1) gene. Analysis of the bla(PER-1) flanking regions showed identity to transposon Tn1213 downstream and 1406 bp upstream of bla(PER-1). Further upstream, an orfA gene and ISPa12 were identified; both were truncated by the insertion of IS6100.
Conclusions: This study confirmed the presence of PER-1-producing P. aeruginosa strains in Greece. The chromosomal location of bla(PER-1), as part of a truncated transposon, suggests clonal expansion rather than horizontal gene transfer.
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http://dx.doi.org/10.1093/jac/dkr471 | DOI Listing |
Eur J Clin Microbiol Infect Dis
December 2024
Faculty of Medicine, Department of Medical Microbiology, Sakarya University, Sakarya, Turkey.
Objective: This study aimed to evaluate the agreement of different methods with the reference method in the detection of colistin resistance in extensively drug-resistant K. pneumoniae, A. baumannii and P.
View Article and Find Full Text PDFCurr Microbiol
December 2024
School of Bio Science and Technology, VIT University, Vellore, Tamil Nadu, 632014, India.
Pseudomonas aeruginosa is a prevalent nosocomial pathogen and a significant reservoir of antimicrobial resistance genes in residential and built environments. It is also widespread in various indoor and outdoor settings, including sewage, surface waters, soil, recreational waters (both treated and untreated), and industrial effluents. Surveillance efforts for P.
View Article and Find Full Text PDFMicrob Pathog
December 2024
Department of Chemistry, Rasht Branch, Islamic Azad University, Rasht, Iran.
Objectives: In the present study, we investigate the effect of FeO nanoparticles conjugated with ursolic acid (FeONPs@UA) on inhibiting the growth, biofilm-forming ability and efflux pump activity in clinical isolates of Pseudomonas aeruginosa with multiple drug resistance.
Methods: Iron oxide NPs conjugated with ursolic acid (FeONPs@UA) were synthesized. Physicochemical features of the NPs were studied by FT-IR, XRD, EDAX, and TEM.
Cureus
November 2024
Microbiology, Kalinga Institute of Medical Sciences, Bhubaneswar, IND.
Background And Objectives: Stroke-associated pneumonia (SAP) is the aftermath of aspiration of oropharyngeal secretions or stomach content. Mechanical ventilation and lowered immunity and consciousness facilitate the etiopathogenesis of SAP. Antibiotic prophylaxis and repeated culture and sensitivity testing dampen the drug susceptibility patterns of the pathogens.
View Article and Find Full Text PDFFront Cell Infect Microbiol
December 2024
Servicio de Microbiología, Hospital Universitario Ramón y Cajal, Instituto Ramón y Cajal de Investigación Sanitaria (IRYCIS), Madrid, Spain.
Introduction: Murepavadin is an antimicrobial peptide (AMP) in clinical development that selectively targets LptD and whose resistance profile remains unknown. We aimed to explore genomic modifications and consequences underlying murepavadin and/or colistin susceptibility.
Methods: To define genomic mechanisms underlying resistance, we performed two approaches: 1) a genome-wide association study (GWAS) in a clinical collection (n=496), considering >0.
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