Two different lengths of long unique synthetic oligonucleotide probes (37- and 48-mers) specific for human major histocompatibility complex (MHC) class II beta genes were synthesized. These oligonucleotides were utilized to examine factors influencing hybridization specificity. Both probe length and stringency of washing conditions were found to be crucial factors for sequence-specific hybridization.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/0735-0651(90)90031-a | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Decoding the excited-state dynamics of carbonyl-containing carotenoids: Insights from the Ind series.
Biochim Biophys Acta Mol Cell Biol Lipids
January 2025
Department of Applied Chemistry for Environment, School of Biological and Environmental Sciences, Kwansei Gakuin University, 1 Gakuen-Uegahara, Sanda, Hyogo 669-1330, Japan.
Carotenoids are naturally occurring pigments essential for both light-harvesting and photoprotection in photosynthetic processes. Among these, carbonyl-containing carotenoids exhibit distinctive excited state properties due to the presence of intramolecular charge transfer (ICT) in their excited states. In this study, we synthesized a novel family of carotenoid analogs with varying numbers of conjugated double bonds, denoted as the Ind series, and conducted femtosecond pump-probe spectroscopy on these molecules in both acetone and n-hexane.
View Article and Find Full Text PDFExploring the impact of a pain assessment information visualization tool on provider-patient pain discussion with limited English proficiency Hmong patients.
Patient Educ Couns
January 2025
University of Wisconsin-Madison, School of Pharmacy, Madison, WI, USA.
Objective: This pilot study examines the impact of the Pain Assessment Information Visualization (InfoViz) Tool on the length of the patient visit for Hmong patients with limited English proficiency (LEP).
Methods: A static design study was used to collect data from a triad of 20 Hmong patients with LEP, medical interpreters, and clinicians in the usual care group followed by 20 triads in the intervention group in primary care. We analyzed patient-clinician communication audio-recorded visits in primary care, examining both the length of the visit and the nature of the clinician efforts to gather more information using Svarstad's clinician conversation conceptualization to explore probing frequencies for three pain categories: pain location, severity, and quality in the intervention group (Pain InfoViz Tool) and usual care group.
Sensitive fluorescent detection of SARS-CoV-2 RNA using an enzymatic-based method.
Spectrochim Acta A Mol Biomol Spectrosc
January 2025
CICS-UBI - Health Sciences Research Centre University of Beira Interior Covilhã Portugal; RISE-Health, Departamento de Química, Faculdade de Ciências, Universidade da Beira Interior, Rua Marquês d'Ávila e Bolama 6201-001 Covilhã, Portugal; Departamento de Química, Universidade da Beira Interior, Rua Marquês de Ávila e Bolama 6201-001 Covilhã, Portugal. Electronic address:
Rapid, quantitative, and sensitive detection of viral oligonucleotides can help to diagnose the infection before symptoms occur, monitor disease progression, and identify viral subtypes. A one-pot, simple, rapid hairpin-mediated nicking enzymatic signal amplification (HNESA) method was previously developed for nucleic acids detection. In the present work, this method was applied for the detection of SARS-CoV-2 RNA by designing an assistant probe (AP) that contains the complementary sequence for the target, the sequence of hybridization with the loop region of the molecular beacon (MB), and the recognition site of the nicking endonuclease Nt.
View Article and Find Full Text PDFChromosome length genome assembly of the stone marten (Martes foina, Mustelidae): a new view on one of the cornerstones in carnivore cytogenetics.
J Hered
January 2025
Center for Evolutionary Hologenomics, The Globe Institute, The University of Copenhagen, 5A, Oester Farimagsgade, Copenhagen, 1353, Denmark.
The stone marten (Martes foina) is an important species for cytogenetic studies in the order Carnivora. ZooFISH probes created from its chromosomes provided a strong and clean signal in chromosome painting experiments and were valuable for studying the evolution of carnivoran genome architecture. The research revealed that the stone marten chromosome set is similar to the presumed ancestral karyotype of the Carnivora, which added an additional value for the species.
View Article and Find Full Text PDFProbing Critical States of Matter on a Digital Quantum Computer.
Phys Rev Lett
December 2024
Quantinuum, 303 S. Technology Court, Broomfield, Colorado 80021, USA.
Although quantum mechanics underpins the microscopic behavior of all materials, its effects are often obscured at the macroscopic level by thermal fluctuations. A notable exception is a zero-temperature phase transition, where scaling laws emerge entirely due to quantum correlations over a diverging length scale. The accurate description of such transitions is challenging for classical simulation methods of quantum systems, and is a natural application space for quantum simulation.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!