We isolated DNA from porcine hair roots by silica matrix. Purified DNA is suitable for PCR analysis of malignant hyperthermia in the pig breeds. The using of hair roots as a source of DNA provides noninvasivity and it is also convenient for breeders. We compared amplification of DNA purified by silica matrix and Prep-A-gene kit. DNA purification by silica is as effective as Prep-A-gene purification and it is advantageous because of the low price.

Download full-text PDF

Source
http://dx.doi.org/10.1016/s0309-1740(98)00127-2DOI Listing

Publication Analysis

Top Keywords

hair roots
12
dna purification
8
malignant hyperthermia
8
porcine hair
8
silica matrix
8
dna
6
simple inexpensive
4
inexpensive dna
4
purification malignant
4
hyperthermia pcr
4

Similar Publications

BcWRKY25-BcWRKY33A-BcLRP1/BcCOW1 module promotes root development for improved salt tolerance in Bok choy.

Hortic Res

January 2025

State Key Laboratory of Crop Genetics & Germplasm Enhancement, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops (East China), Ministry of Agriculture and Rural Affairs of China, Engineering Research Center of Germplasm Enhancement and Utilization of Horticultural Crops, Ministry of Education of China, Nanjing Agricultural University, No.1 Weigang Road, Xuanwu District, Nanjing 210095, China.

Root development is a complex process involving phytohormones and transcription factors. Our previous research has demonstrated that is significantly expressed in Bok choy roots under salt stress, and heterologous expression of increases salt tolerance and promotes root development in transgenic . However, the precise molecular mechanisms by which BcWRKY33A governs root development remain elusive.

View Article and Find Full Text PDF

Spider Fungi: New species of and in the aerial rhizomorph web-maker guild in Amazonia.

Fungal Syst Evol

December 2024

Programa de Pós-graduação em Botânica - DIPO 2, Instituto Nacional de Pesquisas da Amazônia - Inpa, Av. André Araújo 2936, 69067-375, Manaus, AM, Brazil.

Rhizomorphs are hair- or wire-like melanized structures with structural differentiation analogous to plant roots that help fungi spread over an area and find food resources. Some species of multiple groups of the and the produce different types of rhizomorphs. In the , the structures are largely found in , particularly in the , , and .

View Article and Find Full Text PDF

Cyclic nucleotide-gated channel 5 (CNGC5), CNGC6, and CNGC9 (CNGC5/6/9 for simplicity) control Arabidopsis root hair (RH) growth by mediating the influx of external Ca to establish and maintain a sharp cytosolic Ca gradient at RH tips. However, the underlying mechanisms for the regulation of CNGCs remain unknown. We report here that calcium dependent protein kinase 1 (CPK1) directly activates CNGC5/6/9 to promote Arabidopsis RH growth.

View Article and Find Full Text PDF

Horses are animals traditionally playing prominent role as both food source and working animals for Kazakh people. Zhabe horses are traditional type of indigenous Kazakh horses characterized by versatility and adaptation to conditions of Central Asia. The present work focuses on examination of genetic structure of Zhabe horses using SNP genotyping with addition of previously published data.

View Article and Find Full Text PDF

The root epidermis of Arabidopsis (Arabidopsis thaliana) consists of two distinct cell types: hair (H) cells and non-hair (N) cells, whose patterning is regulated by a network of genes. Among these, the WEREWOLF (WER) gene, encoding an R2R3 MYB transcription factor, acts as a master regulator by promoting the expression of key downstream genes, such as GLABRA2 and CAPRICE. However, the mechanisms controlling WER expression have remained largely unexplored.

View Article and Find Full Text PDF

Want AI Summaries of new PubMed Abstracts delivered to your In-box?

Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!