Identification of the commencement of aging in relation to the rigor process was determined on lamb loin muscles by injecting a zinc-TRIS solution, which inhibits meat aging, into the muscle over the period when the pH was falling. Aging was evaluated by measuring the tenderness of cooked meat samples using a MIRINZ tenderometer. When zinc was injected into muscle above pH 6·5, the sarcomeres shortened and the cooked meat had an average shear force of 19 kgF (shear force is the force required to shear through at 1 cm cross-section sample). When sinc was injected into the muscle at pH values below 6·5 the sarcomeres did not shorten, and down to the ultimate pH, the shear force remained at approximately 15 kfF. Aging did not take place pre-rigor. The same relationships occurred for electrically stimulated muscle. The data indicate that the basal shear-force value of lamb loin prior to aging, i.e. the basal toughness level, is 15 kgF.
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Int Endod J
January 2025
School of Medicine and Dentistry, Griffith University, Gold Coast, Australia.
Introduction: Biofilms may show varying adherence strengths to dentine. This study quantified the shear force required for the detachment of multispecies biofilm from the dentine using fluid dynamic gauging (FDG) and computation fluid dynamics (CFD). To date this force has not been quantified.
View Article and Find Full Text PDFACS Nano
January 2025
Louvain Institute of Biomolecular Science and Technology, UCLouvain, Croix du Sud, 4-5, bte L7.07.07, B-1348 Louvain-la-Neuve, Belgium.
The iron-regulated surface determinant protein B (IsdB) has recently been shown to bind to toll-like receptor 4 (TLR4), thereby inducing a strong inflammatory response in innate immune cells. Currently, two unsolved questions are (i) What is the molecular mechanism of the IsdB-TLR4 interaction? and (ii) Does it also play a role in nonimmune systems? Here, we use single-molecule experiments to demonstrate that IsdB binds TLR4 with both weak and extremely strong forces and that the mechanostability of the molecular complex is dramatically increased by physical stress, sustaining forces up to 2000 pN, at a loading rate of 10 pN/s. We also show that TLR4 binding by IsdB mediates time-dependent bacterial adhesion to endothelial cells, pointing to the role of this bond in cell invasion.
View Article and Find Full Text PDFSoft Matter
January 2025
Institute of Biochemistry and Biophysics, University of Tehran, Tehran, Iran.
Microfluidic chips are powerful tools for investigating numerous variables including chemical and physical parameters on protein aggregation. This study investigated the aggregation of bovine serum albumin (BSA) in two different systems: a vial-based static system and a microfluidic chip-based dynamic system in which BSA aggregation was induced successfully. BSA aggregation induced in a microfluidic chip on a timescale of seconds enabled a dynamic investigation of the forces driving the aggregation process.
View Article and Find Full Text PDFSci Rep
January 2025
School of Safety Engineering, China University of Mining and Technology, Xuzhou, 221116, China.
The ore mining sites commonly experience slope instability, which is causing concern for the workers' safety and the operation's stability. Considering the Ziluoyi iron ore mining site as a case study, uniaxial compression strength and shear tests are performed on the lower disk peripheral rock, ore body, and upper disk peripheral rock, leading to the extraction of compressive strength and elastic modulus (lower disk: 77.7 MPa-9.
View Article and Find Full Text PDFJ Appl Biomech
January 2025
Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta, GA, USA.
Repetitive manual labor tasks involving twisting, bending, and lifting commonly lead to lower back and knee injuries in the workplace. To identify tasks with high injury risk, we recruited N = 9 participants to perform industry-relevant, 2-handed lifts with a 11-kg weight. These included symmetrical/asymmetrical, ascending/descending lifts that varied in start-to-end heights (knee-to-waist and waist-to-shoulder).
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