AI Article Synopsis
- Freeze-drying (lyophilization) could offer a better way to preserve sperm compared to current cryopreservation methods, addressing issues like high costs and risks associated with transporting frozen samples.
- After freeze-drying, sperm lose motility upon rehydration, which is crucial for fertilization, necessitating a complex technique called microinsemination for successful use.
- Although it's believed that freeze-dried sperm can retain their functional capabilities after being stored at fridge temperatures, achieving effective production of viable offspring from these samples remains a significant challenge, especially in larger farm animals.
Article Abstract
Freeze-drying (lyophilization) has been proposed as an alternative method for sperm preservation to overcome the disadvantages of the current cryopreservation method such as the high maintenance cost of frozen stocks, the problems associated with transportation of frozen materials and the potential risk of total loss of the frozen stock. Since freeze-dried spermatozoa after rehydration lose their motility, which is an essential requirement to complete physiological fertilization, a relatively difficult microinsemination technique must be applied to rehydrated spermatozoa. Theoretically, it has been supposed that freeze-dried spermatozoa could maintain their functions and abilities to interact with the oocyte cytoplasm after prolonged storage at refrigerator temperature. However, sufficient yield of transferable blastocysts and production of live offspring derived from freeze-dried sperm samples are still subjects to be challenged and overcome in large domestic species.
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| http://dx.doi.org/10.1262/jrd.11-061o | DOI Listing |
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