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Developing a novel TaqMan qPCR assay for optimizing Pullorum detection in chickens.

Vet Q

December 2025

Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.

Pullorum, the causative agent of pullorum disease, posing a significant threat to the global production of poultry meat and eggs. However, existing detection methods have substantial limitations in efficiency and accuracy. Herein, we developed a genomic deletion-targeted TaqMan qPCR assay for identification of Pullorum, enabling precise differentiation from other serovars.

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Background: Vaccine hesitancy is a serious public health problem globally, particularly in low- and middle-income countries like Pakistan. This study aims to determine the vaccination refusal rate, associated factors and perceptions of parents who refused routine immunisation within Peshawar, Khyber Pakhtunkhwa, Pakistan.

Methods: A cross-sectional study conducted in July-2024, among 340 parents of children aged 0-59 months.

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Introduction: National malaria programmes must weigh the relative benefits of different vector control and elimination tools to prioritise resource allocation with the greatest impact. This study assesses the epidemiological and entomological impacts of piperonyl butoxide insecticide-treated nets (PBO ITN-only arm) compared with the combination of two annual non-pyrethroid indoor residual spraying (IRS) campaigns and standard pyrethroid ITNs (IRS+Standard Pyrethroid ITN arm) in the Amhara region of Ethiopia.

Methods: An open-label, stratified block-cluster randomised trial was designed to compare the impacts of the two intervention arms.

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On the ethical governance of swarm robotic systems in the real world.

Philos Trans A Math Phys Eng Sci

January 2025

Bristol Robotics Laboratory, School of Engineering Mathematics and Technology, University of Bristol, Bristol BS8 1TW, UK.

In this paper, we address the question: what practices would be required for the responsible design and operation of real-world swarm robotic systems? We argue that swarm robotic systems must be developed and operated within a framework of ethical governance. We will also explore the human factors surrounding the operation and management of swarm systems, advancing the view that human factors are no less important to swarm robots than social robots. Ethical governance must be anticipatory, and a powerful method for practical anticipatory governance is ethical risk assessment (ERA).

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