AI Article Synopsis
- Counterselectable cassettes are valuable tools in molecular biology for making deletion mutants or point mutations without visible markers.
- The author developed an inducible sacB cassette that operates with a tetracycline repressor to enhance precision in genetic manipulation.
- When paired with a kanamycin-resistance marker, this system effectively created unmarked mutants in Haemophilus influenzae while minimizing typical issues related to sacB use in counterselection.
Article Abstract
Counterselectable cassettes are extremely useful in molecular biology and allow for the creation of unmarked deletion mutants or the introduction of point mutations. I have constructed an inducible sacB cassette, using the tetracycline repressor. When used in tandem with a kanamycin-resistance marker, the cassette was successful in creating unmarked mutants in Haemophilus influenzae. The inducible nature of the cassette avoids some of the common problems associated with the utilization of sacB in counterselection.
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| http://dx.doi.org/10.1016/j.gene.2011.10.014 | DOI Listing |
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