Surfaces presenting a heparin-binding peptide can non-covalently sequester heparin from culture supplements, such as fetal bovine serum. In turn, sequestered, serum-borne heparin can non-covalently localize growth factors at the cell-material interface, resulting in amplified growth factor bioactivity.
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http://dx.doi.org/10.1002/adma.201103046 | DOI Listing |
Front Cell Infect Microbiol
September 2017
Vector-Borne Diseases Laboratories, Department of Pathobiological Sciences, Louisiana State University School of Veterinary Medicine Baton Rouge, LA, USA.
Spotted fever group (SFG) species are inoculated into the mammalian bloodstream by hematophagous arthropods. Once in the bloodstream and during dissemination, the survival of these pathogens is dependent upon the ability of these bacteria to evade serum-borne host defenses until a proper cellular host is reached. expresses an outer membrane protein, Adr1, which binds the complement inhibitory protein vitronectin to promote resistance to the anti-bacterial effects of the terminal complement complex.
View Article and Find Full Text PDFBiomacromolecules
June 2014
Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, Wisconsin 53706, United States.
Vascular endothelial growth factor (VEGF) activity is highly regulated via sequestering within the ECM and cell-demanded proteolysis to release the sequestered VEGF. Numerous studies have demonstrated that VEGF activity mediates cellular events leading to angiogenesis and capillary formation in vivo. This has motivated the study of biomaterials to sustain VEGF release, and in many cases, the materials are inspired by the structure and function of the native ECM.
View Article and Find Full Text PDFAdv Mater
December 2011
Department of Biomedical Engineering, University of Wisconsin-Madison, 53705, USA.
Surfaces presenting a heparin-binding peptide can non-covalently sequester heparin from culture supplements, such as fetal bovine serum. In turn, sequestered, serum-borne heparin can non-covalently localize growth factors at the cell-material interface, resulting in amplified growth factor bioactivity.
View Article and Find Full Text PDFIntegr Biol (Camb)
August 2011
Department of Biomedical Engineering, University of Wisconsin, 2130 Engineering Centers Building, 1550 Engineering Drive, Madison, WI 53706, USA.
The influence of specific serum-borne biomolecules (e.g. heparin) on growth factor-dependent cell behavior is often difficult to elucidate in traditional cell culture due to the random, non-specific nature of biomolecule adsorption from serum.
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