The tandem starch-binding domains (KvSBD) located at carboxy-terminal region of halophilic α-amylase from moderate halophile, Kocuria varians, were expressed in E. coli with amino-terminal hexa-His-tag and purified to homogeneity. The recombinant KvSBD showed binding activity to raw starch granules at low to high salt concentrations. The binding activity of KvSBD to starch was fully reversible after heat-treatment at 85°C. Circular dichroism and thermal scanning experiments indicated that KvSBD showed fully reversible refolding upon cooling after complete melting at 70°C in the presence of 0.2-2.0M NaCl. The refolding rate was enhanced with higher salt concentration.
Download full-text PDF |
Source |
|---|---|
| http://dx.doi.org/10.1016/j.ijbiomac.2011.10.007 | DOI Listing |
Publication Analysis
Top Keywords
Similar Publications
Biocide Tolerance and Impact of Sanitizer Concentrations on the Antibiotic Resistance of Bacteria Originating from Cheese.
Foods
October 2023
Department of Food Science, Faculty of Economics, Socio-Human Sciences and Engineering, Sapientia Hungarian University of Transylvania, 530104 Miercurea Ciuc, Romania.
In this study, we determined and identified the bacterial diversity of different types of artisanal and industrially produced cheese. The antibiotic (erythromycin, chloramphenicol, kanamycin, ampicillin, clindamycin, streptomycin, tetracycline, and gentamicin) and biocide (peracetic acid, sodium hypochlorite, and benzalkonium chloride) resistance of clinically relevant bacteria was determined as follows: , , sp., , , , , , , , sp.
View Article and Find Full Text PDFKocuria species: an underappreciated pathogen in pediatric patients-a single-center retrospective analysis of 10 years' experience in China.
Diagn Microbiol Infect Dis
December 2023
Department of Nephrology, Rheumatology and Immunology, Hunan Children's Hospital, Changsha, Hunan, China; The School of Pediatrics, Hengyang Medical School, University of South China, Hengyang, Hunan, China. Electronic address:
Kocuria species are known to be opportunistic pathogens that cause infections in humans, especially immunocompromised hosts. However, reports of pediatric patients are limited. This retrospective study was designed to investigate the spectrum of infections in pediatric patients caused by Kocuria species.
View Article and Find Full Text PDFDynamic interspecies interactions and robustness in a four-species model biofilm.
Microbiologyopen
November 2021
INRAE, AgroParisTech, Micalis Institute, Université Paris-Saclay, Jouy-en-Josas, France.
Interspecific interactions within biofilms determine relative species abundance, growth dynamics, community resilience, and success or failure of invasion by an extraneous organism. However, deciphering interspecific interactions and assessing their contribution to biofilm properties and function remain a challenge. Here, we describe the constitution of a model biofilm composed of four bacterial species belonging to four different genera (Rhodocyclus sp.
View Article and Find Full Text PDFA Rare Case of Pediatric Central Venous Catheter-Related Bloodstream Infection With Kocuria Varians.
Cureus
September 2021
Infectious Diseases, Al Jalila Children's Specialty Hospital, Dubai, ARE.
Cases of central line blood infections caused by spp. are limited in the literature. Most of those infections have been detected in hospitalized patients with severe underlying disease or those with implanted catheters or with suppressed immunity.
View Article and Find Full Text PDFFour species of bacteria deterministically assemble to form a stable biofilm in a millifluidic channel.
NPJ Biofilms Microbiomes
August 2021
Sorbonne Université, CNRS, Laboratoire Jean Perrin (UMR 8237), 4 place Jussieu, F-75005, Paris, France.
Multispecies microbial adherent communities are widespread in nature and organisms, although the principles of their assembly and development remain unclear. Here, we test the possibility of establishing a simplified but relevant model of multispecies biofilm in a non-invasive laboratory setup for the real-time monitoring of community development. We demonstrate that the four chosen species (Bacillus thuringiensis, Pseudomonas fluorescens, Kocuria varians, and Rhodocyclus sp.
View Article and Find Full Text PDFWant AI Summaries of new PubMed Abstracts delivered to your In-box?
Enter search terms and have AI summaries delivered each week - change queries or unsubscribe any time!