AI Article Synopsis
- The study aimed to assess the cytotoxic effects of the self-etch resin-based luting cement, RelyXUnicem, under different curing conditions, with or without the placement of ceramic discs.
- Researchers created seven groups of specimens to evaluate the impact on cultured odontoblast-like cells, measuring indicators like cell metabolism, total protein dosage, and alkaline phosphatase activity after exposure to cement extracts.
- Results indicated that the chemical curing process without ceramic interposition led to mild cytotoxic effects, while dual curing without ceramic showed no cytotoxicity; significant cell metabolism and protein reductions were noted with certain groups.
Article Abstract
Purpose: To evaluate the cytotoxicity of a self-etch resin-based luting cement, RelyXUnicem (RXU) upon chemical or dual cure and with or without interposition of IPS d.SIGN (IPSD) or IPS Empress II (IPSE) ceramic discs between cement and light source.
Methods: 112 RXU specimens were subjected to different curing conditions and incubated in culture medium (DMEM) to obtain extracts. The following groups were formed: G1: DMEM (control); G2: dual RXU; G3: chemical RXU; G4: dual RXU+IPSD; G5: chemical RXU+IPSD; G6: dual RXU+IPSE; and G7: chemical RXU+IPSE. Cultured odontoblast-like cells were incubated for 24 hours in contact with the extracts. Data from cell metabolism (CM), total protein dosage (TPD) and alkaline phosphatase activity (APA) were obtained and analyzed statistically (alpha = 0.05; Kruskal Wallis and Mann-Whitney tests). Cell morphology was analyzed by SEM.
Results: CM and APA were significantly lower in G3 and G7 than in G1 (P<0.05). Significant TPD decrease occurred in G5 and G7 compared to G1 (P<0.05). Only G4 and G6 presented CM changes. RXU caused no cytotoxicity when subjected to dual cure without ceramic interposition. However, mild cytopathic effects were observed after chemical setting without ceramic interposition, and after chemical and dual activation under ceramic discs.
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